Flow inconsistency: The evil twin of column switching-Hardware aspects

Eduard Rogatsky; Kara Braaten; Greg Cruikshank; Harsha Jayatillake; Bing Na Zheng; Daniel T. Stein

doi:10.1016/j.chroma.2009.08.078

Flow inconsistency: The evil twin of column switching-Hardware aspects

Eduard Rogatsky, Kara Braaten, Greg Cruikshank, Harsha Jayatillake, Bing Na Zheng, Daniel T. Stein

Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.

Original language	English (US)
Pages (from-to)	7721-7727
Number of pages	7
Journal	Journal of Chromatography A
Volume	1216
Issue number	45
DOIs	https://doi.org/10.1016/j.chroma.2009.08.078
State	Published - Nov 6 2009

Keywords

Column switching
Dead (delay) volume
Flow inconsistency
Focus mode
Pressurization
Turbulent flow chromatography

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2009.08.078

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title = "Flow inconsistency: The evil twin of column switching-Hardware aspects",

abstract = "Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.",

keywords = "Column switching, Dead (delay) volume, Flow inconsistency, Focus mode, Pressurization, Turbulent flow chromatography",

author = "Eduard Rogatsky and Kara Braaten and Greg Cruikshank and Harsha Jayatillake and Zheng, {Bing Na} and Stein, {Daniel T.}",

note = "Funding Information: This research was supported in part by a Clinical and Translational Science Award (CTSA) from the National Institutes of Health ( UL11RR025740 ) awarded to the Albert Einstein College of Medicine of Yeshiva University. ",

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AU - Rogatsky, Eduard

AU - Braaten, Kara

AU - Cruikshank, Greg

AU - Jayatillake, Harsha

AU - Zheng, Bing Na

AU - Stein, Daniel T.

N1 - Funding Information: This research was supported in part by a Clinical and Translational Science Award (CTSA) from the National Institutes of Health ( UL11RR025740 ) awarded to the Albert Einstein College of Medicine of Yeshiva University.

PY - 2009/11/6

Y1 - 2009/11/6

N2 - Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.

AB - Solvent flow, generated by HPLC pumps is consistent and accurate. This statement, while true for single column (one dimensional) liquid chromatography applications, may not apply to column switching applications. Connection of pumps and/or columns to one flow path may cause substantial pressure changes. Immediate post valve switch pressure differences between pumps can cause backflow where the mobile phase stored at higher pressure will temporary flow into the lower pressure area. A more common side effect of column switching is flow inconsistency during pump pressurization. For the duration of pump pressurization, liquid flow through the column will be smaller than expected since the HPLC column acts like a flow restrictor.

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Flow inconsistency: The evil twin of column switching-Hardware aspects

Abstract

Keywords

ASJC Scopus subject areas

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