Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Francesca Pennacchietti; Ekaterina O. Serebrovskaya; Aline R. Faro; Irina I. Shemyakina; Nina G. Bozhanova; Alexey A. Kotlobay; Nadya G. Gurskaya; Andreas Bodén; Jes Dreier; Dmitry M. Chudakov; Konstantin A. Lukyanov; Vladislav V. Verkhusha; Alexander S. Mishin; Ilaria Testa

doi:10.1038/s41592-018-0052-9

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Francesca Pennacchietti
, Ekaterina O. Serebrovskaya
, Aline R. Faro
, Irina I. Shemyakina
, Nina G. Bozhanova
, Alexey A. Kotlobay
, Nadya G. Gurskaya
, Andreas Bodén
, Jes Dreier
, Dmitry M. Chudakov
, Konstantin A. Lukyanov
, Vladislav V. Verkhusha
, Alexander S. Mishin
, Ilaria Testa

Genetics

Research output: Contribution to journal › Article › peer-review

82 Scopus citations

Abstract

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

Original language	English (US)
Pages (from-to)	601-604
Number of pages	4
Journal	Nature Methods
Volume	15
Issue number	8
DOIs	https://doi.org/10.1038/s41592-018-0052-9
State	Published - Aug 1 2018

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Pennacchietti, F., Serebrovskaya, E. O., Faro, A. R., Shemyakina, I. I., Bozhanova, N. G., Kotlobay, A. A., Gurskaya, N. G., Bodén, A., Dreier, J., Chudakov, D. M., Lukyanov, K. A., Verkhusha, V. V., Mishin, A. S., & Testa, I. (2018). Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy. Nature Methods, 15(8), 601-604. https://doi.org/10.1038/s41592-018-0052-9

Pennacchietti, F, Serebrovskaya, EO, Faro, AR, Shemyakina, II, Bozhanova, NG, Kotlobay, AA, Gurskaya, NG, Bodén, A, Dreier, J, Chudakov, DM, Lukyanov, KA, Verkhusha, VV, Mishin, AS & Testa, I 2018, 'Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy', Nature Methods, vol. 15, no. 8, pp. 601-604. https://doi.org/10.1038/s41592-018-0052-9

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abstract = "Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.",

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AU - Bozhanova, Nina G.

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AU - Bodén, Andreas

AU - Dreier, Jes

AU - Chudakov, Dmitry M.

AU - Lukyanov, Konstantin A.

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AU - Mishin, Alexander S.

AU - Testa, Ilaria

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Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy

Abstract

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