Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1

Julie Dumonceaux; Emmanuel G. Cormier; Francis Kajumo; Gerald P. Donovan; Jayanta Roy-Chowdhury; Ira J. Fox; Jason P. Gardner; Tatjana Dragic

doi:10.1128/JVI.77.24.13418-13424.2003

Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1

Julie Dumonceaux
, Emmanuel G. Cormier
, Francis Kajumo
, Gerald P. Donovan
, Jayanta Roy-Chowdhury
, Ira J. Fox
, Jason P. Gardner
, Tatjana Dragic

Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry assays as well as for budding and virus particle formation.

Original language	English (US)
Pages (from-to)	13418-13424
Number of pages	7
Journal	Journal of virology
Volume	77
Issue number	24
DOIs	https://doi.org/10.1128/JVI.77.24.13418-13424.2003
State	Published - Dec 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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Dumonceaux, J., Cormier, E. G., Kajumo, F., Donovan, G. P., Roy-Chowdhury, J., Fox, I. J., Gardner, J. P., & Dragic, T. (2003). Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1. Journal of virology, 77(24), 13418-13424. https://doi.org/10.1128/JVI.77.24.13418-13424.2003

Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1. / Dumonceaux, Julie; Cormier, Emmanuel G.; Kajumo, Francis et al.
In: Journal of virology, Vol. 77, No. 24, 12.2003, p. 13418-13424.

Research output: Contribution to journal › Article › peer-review

Dumonceaux, J, Cormier, EG, Kajumo, F, Donovan, GP, Roy-Chowdhury, J, Fox, IJ, Gardner, JP & Dragic, T 2003, 'Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1', Journal of virology, vol. 77, no. 24, pp. 13418-13424. https://doi.org/10.1128/JVI.77.24.13418-13424.2003

Dumonceaux J, Cormier EG, Kajumo F, Donovan GP, Roy-Chowdhury J, Fox IJ et al. Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1. Journal of virology. 2003 Dec;77(24):13418-13424. doi: 10.1128/JVI.77.24.13418-13424.2003

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abstract = "Hepatitis C virus (HCV) is a positive-strand RNA virus that replicates exclusively in the cytoplasm of infected cells. The viral envelope glycoproteins, E1 and E2, appear to be retained in the endoplasmic reticulum, where viral budding is thought to occur. Surprisingly, we found that the expression system used to generate HCV envelope glycoproteins influences their subcellular localization and processing. These findings have important implications for optimizing novel HCV fusion and entry assays as well as for budding and virus particle formation.",

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Expression of Unmodified Hepatitis C Virus Envelope Glycoprotein-Coding Sequences Leads to Cryptic Intron Excision and Cell Surface Expression of E1/E2 Heterodimers Comprising Full-Length and Partially Deleted E1

Abstract

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