TY - JOUR
T1 - Evaluation of DNA adduction of AZT in peripheral blood leukocytes of HIV-infected individuals by 32P-post-labeling thin-layer chromatography
T2 - A feasibility study
AU - Huang, Jerry M.
AU - Anastos, Kathryn
AU - Robison, Esther
AU - Shi, Ruijin
AU - Freeman, Kathy
AU - Strickler, Howard
AU - Steinberg, J. J.
PY - 2004/10/15
Y1 - 2004/10/15
N2 - 3′-Azido-3′-deoxythymidine (AZT, Zidovudine) has been effectively used for HIV infection treatment. It inhibits virus reproduction through viral reverse transcriptase inhibition. However, the side effects of this anti-retroviral drug might be cumulative, particularly in its effects on the patients' DNA. As a nucleoside analogue, AZT might incorporate into hosts' DNA, and then form DNA adducts. This may result in potential long-term risks of mutagenesis in AIDS patients who received therapy. In this feasibility study, a 32P-post-labeling thin-layer chromatography (TLC) assay is successfully used to measure AZT-DNA analogue and adducts formed in peripheral blood leukocytes of AZT treated patients. There are DNA analogue/adducts measured in all four AZT treated patients' DNA specimens. This assay is reliable with the significant coefficient of correlation in both intra-assay (r = 0.8761, P = 0.0001) and inter-assay (r = 0.8761, P = 0.0001).
AB - 3′-Azido-3′-deoxythymidine (AZT, Zidovudine) has been effectively used for HIV infection treatment. It inhibits virus reproduction through viral reverse transcriptase inhibition. However, the side effects of this anti-retroviral drug might be cumulative, particularly in its effects on the patients' DNA. As a nucleoside analogue, AZT might incorporate into hosts' DNA, and then form DNA adducts. This may result in potential long-term risks of mutagenesis in AIDS patients who received therapy. In this feasibility study, a 32P-post-labeling thin-layer chromatography (TLC) assay is successfully used to measure AZT-DNA analogue and adducts formed in peripheral blood leukocytes of AZT treated patients. There are DNA analogue/adducts measured in all four AZT treated patients' DNA specimens. This assay is reliable with the significant coefficient of correlation in both intra-assay (r = 0.8761, P = 0.0001) and inter-assay (r = 0.8761, P = 0.0001).
KW - 3′-Azido-3′-deoxythymidine
KW - DNA
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U2 - 10.1016/S1570-0232(04)00538-0
DO - 10.1016/S1570-0232(04)00538-0
M3 - Article
C2 - 15358301
AN - SCOPUS:4444221473
SN - 1570-0232
VL - 810
SP - 1
EP - 6
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -