TY - JOUR
T1 - Electrolytic reduction of carrier-free 188Re
AU - Dadachova, Ekaterina
AU - Smith, Suzanne V.
AU - Mirzadeh, Saed
N1 - Funding Information:
Acknowledgements--Research at ANSTO was supported by the Commonwealth of Australia. Research at ORNL was supported by the OHER, U.S. Department of Energy, under Contract DE-AC05-840R21400 with Martin Marietta Energy Systems, Inc.
PY - 1996/3
Y1 - 1996/3
N2 - Electrolytic reduction of 188Re7+ as an alternative to reduction with SnCl2 for protein labeling has been investigated. The electrolysis cell consisted of an "H" type cell with a tungsten cathode and a platinum anode, and 7 M HCl as the supporting electrolyte. Reduction of 188ReO4- was conducted at 25-35 V (I = 0.05-0.5 A, current density on cathode 4 × 10-3-4 × 10-2 A·cm-2) for 15 min. Ascending paper chromatography on Whatman DE81 paper in 7 M HCl at 4°C confirmed that 75-77% of 188Re was reduced to the + 5 oxidation state. Subsequent to evaporation of 7 M HCl under nitrogen, it was shown that the reduced 188ReS+ as complexed with DMSA (meso-1,2-dimercaptosuccinic acid) or citrate at pH = 4.5 was stable with regard to reoxidation for up to 40 min.
AB - Electrolytic reduction of 188Re7+ as an alternative to reduction with SnCl2 for protein labeling has been investigated. The electrolysis cell consisted of an "H" type cell with a tungsten cathode and a platinum anode, and 7 M HCl as the supporting electrolyte. Reduction of 188ReO4- was conducted at 25-35 V (I = 0.05-0.5 A, current density on cathode 4 × 10-3-4 × 10-2 A·cm-2) for 15 min. Ascending paper chromatography on Whatman DE81 paper in 7 M HCl at 4°C confirmed that 75-77% of 188Re was reduced to the + 5 oxidation state. Subsequent to evaporation of 7 M HCl under nitrogen, it was shown that the reduced 188ReS+ as complexed with DMSA (meso-1,2-dimercaptosuccinic acid) or citrate at pH = 4.5 was stable with regard to reoxidation for up to 40 min.
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U2 - 10.1016/0969-8043(95)00301-0
DO - 10.1016/0969-8043(95)00301-0
M3 - Article
AN - SCOPUS:0029873730
SN - 0969-8043
VL - 47
SP - 289
EP - 292
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 3
ER -