Abstract
Objective: To study the effect and associated molecular mechanism of miR-23a on the chondrogenic differentiation of rat bone marrow derived mesenchymal stem cells(BMSCs).
Methods:BMSCs were isolated from adult rats and then transfected with miR-23a. Chondrogenic differentiation associated genes were then analyzed by RT-PCR. Chondroitin sulfate expression was analyzed by sarranine O staining. Sox9 and Run2 protein expressions were evaluated by Western blot analysis.
Results:BMSCs transfected with miR-23a showed a higher level of chondrogenic differentiation related genes such as Sox9, Collagen II and Aggrecan. The expression of chondrocyte hypertrophy related genes Collagen X was lower in the miR-23a transfected group than the control group. Chondroitin sulfate expression was also elevated in the miR-23a transfected group than the control group as shown by sarranine O staining. Finally, Sox9 protein expression was increased in the miR-23a transfected group and Runx2 protein expression was decreased in the miR-23a transfected group as demonstrated by Western blot analysis.
Conclusion:miR-23a promotes chondrogenic differentiation and inhibits hypertrophy of BMSCs by increasing Sox9 expression and decreasing Runx2 expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 493-497 |
| Number of pages | 5 |
| Journal | Medical Journal of Wuhan University |
| Volume | 35 |
| Issue number | 4 |
| State | Published - Jul 15 2014 |
| Externally published | Yes |
Keywords
- Bone Marrow Derived Mesenchymal Stem Cells
- Chondrogenic Differentiation
- Hyperthophy
- MicroRNA-23a
ASJC Scopus subject areas
- General Medicine
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