TY - JOUR
T1 - Dynein arm substructure and the orientation of arm-microtubule attachments
AU - Avolio, Jock
AU - Lebduska, Stephen
AU - Satir, Peter
N1 - Funding Information:
We thank William Reed and Timothy Otter for helpful discussion. This work was supported by grants HL22560 and GM27859 from the United States Public Service.
PY - 1984/3/5
Y1 - 1984/3/5
N2 - In the presence of AMP-PCP (β,γ-methyleneadenosine 5′-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arrangement of these subunits makes it possible to distinguish A from B subfiber binding sites on a single arm and to demonstrate that the head of an extended arm on subfiber A of one ciliary doublet is capable of binding to subfiber B of an adjacent doublet in a specific orientation, which supports a key step in a current model of the mechanochemical cycle by which the arm produces microtubule sliding in the ciliary axoneme.
AB - In the presence of AMP-PCP (β,γ-methyleneadenosine 5′-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arrangement of these subunits makes it possible to distinguish A from B subfiber binding sites on a single arm and to demonstrate that the head of an extended arm on subfiber A of one ciliary doublet is capable of binding to subfiber B of an adjacent doublet in a specific orientation, which supports a key step in a current model of the mechanochemical cycle by which the arm produces microtubule sliding in the ciliary axoneme.
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U2 - 10.1016/0022-2836(84)90127-X
DO - 10.1016/0022-2836(84)90127-X
M3 - Article
C2 - 6230460
AN - SCOPUS:0021755425
SN - 0022-2836
VL - 173
SP - 389
EP - 401
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 3
ER -