Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum

Menno L.W. Knetsch; Stefan J.P. Epskamp; Paul W. Schenk; Yiwen Wang; Jeffrey E. Segall; B. Ewa Snaar-Jagalska

Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum

Menno L.W. Knetsch, Stefan J.P. Epskamp, Paul W. Schenk, Yiwen Wang, Jeffrey E. Segall, B. Ewa Snaar-Jagalska

Anatomy & Structural Biology

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.

Original language	English (US)
Pages (from-to)	3361-3368
Number of pages	8
Journal	EMBO Journal
Volume	15
Issue number	13
State	Published - Jul 1 1996

Keywords

Cross-talk
Dictyostelium
G-protein
MAP kinase
ras

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

Cite this

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title = "Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum",

abstract = "Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.",

keywords = "Cross-talk, Dictyostelium, G-protein, MAP kinase, ras",

author = "Knetsch, {Menno L.W.} and Epskamp, {Stefan J.P.} and Schenk, {Paul W.} and Yiwen Wang and Segall, {Jeffrey E.} and Snaar-Jagalska, {B. Ewa}",

year = "1996",

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AU - Knetsch, Menno L.W.

AU - Epskamp, Stefan J.P.

AU - Schenk, Paul W.

AU - Wang, Yiwen

AU - Segall, Jeffrey E.

AU - Snaar-Jagalska, B. Ewa

PY - 1996/7/1

Y1 - 1996/7/1

N2 - Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.

AB - Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.

KW - Cross-talk

KW - Dictyostelium

KW - G-protein

KW - MAP kinase

KW - ras

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Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum

Abstract

Keywords

ASJC Scopus subject areas

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