@article{a815fcfefd0b442a8d601bd4e67526f3,
title = "Donor-derived vasculature is required to support neocortical cell grafts after stroke",
abstract = "Neural precursor cells (NPCs) transplanted into the adult neocortex generate neurons that synaptically integrate with host neurons, supporting the possibility of achieving functional tissue repair. However, poor survival and functional neuronal recovery of transplanted NPCs greatly limits engraftment. Here, we test the hypothesis that combining blood vessel-forming vascular cells with neuronal precursors improves engraftment. By transplanting mixed embryonic neocortical cells into adult mice with neocortical strokes, we show that transplant-derived neurons synapse with appropriate targets while donor vascular cells form vessels that fuse with the host vasculature to perfuse blood within the graft. Although all grafts became vascularized, larger grafts had greater contributions of donor-derived vessels that increased as a function of their distance from the host-graft border. Moreover, excluding vascular cells from the donor cell population strictly limited graft size. Thus, inclusion of vessel-forming vascular cells with NPCs is required for more efficient engraftment and ultimately for tissue repair.",
keywords = "Blood vessels, Ischemia, Neocortex, Transplantation, Vascular endothelial cells",
author = "Joanna Krzyspiak and Jingqi Yan and Ghosh, {Hiyaa S.} and Basia Galinski and Lituma, {Pablo J.} and Karina Alvina and Alexandra Quezada and Samantha Kee and Marta Gro{\'n}ska-P{\c e}ski and Tai, {Yi De} and Kelsey McDermott and Gon{\c c}alves, {J. Tiago} and Zukin, {R. Suzanne} and Weiser, {Daniel A.} and Castillo, {Pablo E.} and Kamran Khodakhah and H{\'e}bert, {Jean M.}",
note = "Funding Information: We are grateful for grant support from Aubrey de Grey and the SENS Research Foundation (JMH), New York State Department of Health NYSTEM Program for shared facility grant support C029154 (JMH), NYSTEM C34875GG (JMH), NYSTEM Einstein Training Program in Stem Cell Research C30292GG (JK and MGP), Brain Research Foundation (JMH), NIH NS088943 (JMH) and MH125772 (PEC), Ruth L. Kirschstein NRSA Fellowship F31MH109267 (PJL), as well as seed funding from the Albert Einstein College of Medicine. We are also grateful to lab members for feedback on the manuscript. Funding Information: Jean M. Hebert reports financial support was provided by New York State-Department of Health, NYSTEM program. Joanna Krzyspiak reports financial support was provided by New York State-Department of Health, NYSTEM program. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2022",
month = mar,
doi = "10.1016/j.scr.2021.102642",
language = "English (US)",
volume = "59",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
}