DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Masako Suzuki; John M. Greally

doi:10.1016/j.ymeth.2010.04.013

DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Masako Suzuki, John M. Greally

Genetics

Research output: Contribution to journal › Review article › peer-review

23 Scopus citations

Abstract

The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

Original language	English (US)
Pages (from-to)	218-222
Number of pages	5
Journal	Methods
Volume	52
Issue number	3
DOIs	https://doi.org/10.1016/j.ymeth.2010.04.013
State	Published - Nov 2010

Keywords

CpG island
Cytosine methylation
Epigenetic

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ymeth.2010.04.013

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abstract = "The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.",

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AB - The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

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KW - Epigenetic

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JO - Methods

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DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Abstract

Keywords

ASJC Scopus subject areas

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