Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

E. M. Oltz; S. Pollack; T. Delohery; M. J. Smith; M. Ojika; S. Lee; K. Kustin; K. Nakanishi

doi:10.1007/BF01954871

Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

E. M. Oltz, S. Pollack, T. Delohery, M. J. Smith, M. Ojika, S. Lee, K. Kustin, K. Nakanishi

Medicine

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.

Original language	English (US)
Pages (from-to)	186-190
Number of pages	5
Journal	Experientia
Volume	45
Issue number	2
DOIs	https://doi.org/10.1007/BF01954871
State	Published - Feb 1989

Keywords

Fluorescence activated cell sorting (FACS)
morula cells
signet ring cells
tunicates
vanadium

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01954871

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title = "Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry",

abstract = "Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.",

keywords = "Fluorescence activated cell sorting (FACS), morula cells, signet ring cells, tunicates, vanadium",

author = "Oltz, {E. M.} and S. Pollack and T. Delohery and Smith, {M. J.} and M. Ojika and S. Lee and K. Kustin and K. Nakanishi",

year = "1989",

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language = "English (US)",

volume = "45",

pages = "186--190",

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T1 - Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

AU - Oltz, E. M.

AU - Pollack, S.

AU - Delohery, T.

AU - Smith, M. J.

AU - Ojika, M.

AU - Lee, S.

AU - Kustin, K.

AU - Nakanishi, K.

PY - 1989/2

Y1 - 1989/2

N2 - Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.

AB - Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.

KW - Fluorescence activated cell sorting (FACS)

KW - morula cells

KW - signet ring cells

KW - tunicates

KW - vanadium

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Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

Abstract

Keywords

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