TY - CHAP
T1 - Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure Using Laser Capture Microdissection
AU - Gutierrez-Chavez, Claudia
AU - Knockaert, Samantha
AU - Dieu-Nosjean, Marie Caroline
AU - Goc, Jérémy
N1 - Funding Information:
We thank all present and past members of the Laboratory “Cancer, Immune control and Escape” (UMRS1138 INSERM, Cordeliers Research Center), pathologists, and clinicians who have participated in these studies, for their help and valuable discussions. We are grateful to lung cancer patients involved in this research performed in our laboratory. Funding: This work was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM), Pierre and Marie Curie University, Paris-Descartes University, the Labex Immuno-Oncology (LAXE62_9UMRS872 Fridman), CARPEM (Cancer Research for PErsonalized Medicine), Fondation ARC pour la Recherche sur le Cancer. Jeremy Goc was supported by a grant from the Fondation ARC pour la Recherche sur le Cancer.
Publisher Copyright:
© 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018
Y1 - 2018
N2 - Tertiary lymphoid structures (TLS) are de novo lymphoid formations that are induced within tissues during inflammatory episodes. TLS have been reported at various anatomic sites and in many different contexts like cancer, infections, autoimmunity, graft rejection, and idiopathic diseases. These inducible, ectopic, and transient lymphoid structures exhibit the prototypical architecture found within secondary lymphoid organs (SLO) and have been recently appreciated as a major driver of the local adaptive immune reaction. As TLS emerge within tissues, the isolation in situ and the molecular characterization of these structures are challenging to operate. Laser capture microdissection (LCM) is a powerful tool to isolate selective structural components and cells from frozen or paraffin-embedded tissues. We and other groups previously applied LCM to decipher the molecular network within TLS and uncover their intrinsic connection with the local microenvironment. In this chapter, we describe a detailed LCM method for selecting and isolating TLS in situ to perform comprehensive downstream molecular analyses.
AB - Tertiary lymphoid structures (TLS) are de novo lymphoid formations that are induced within tissues during inflammatory episodes. TLS have been reported at various anatomic sites and in many different contexts like cancer, infections, autoimmunity, graft rejection, and idiopathic diseases. These inducible, ectopic, and transient lymphoid structures exhibit the prototypical architecture found within secondary lymphoid organs (SLO) and have been recently appreciated as a major driver of the local adaptive immune reaction. As TLS emerge within tissues, the isolation in situ and the molecular characterization of these structures are challenging to operate. Laser capture microdissection (LCM) is a powerful tool to isolate selective structural components and cells from frozen or paraffin-embedded tissues. We and other groups previously applied LCM to decipher the molecular network within TLS and uncover their intrinsic connection with the local microenvironment. In this chapter, we describe a detailed LCM method for selecting and isolating TLS in situ to perform comprehensive downstream molecular analyses.
KW - Frozen tissue
KW - Gene expression profiling
KW - Laser capture microdissection
KW - Lung cancer
KW - Lymphocyte
KW - RNA analysis
KW - Tertiary lymphoid structure
UR - http://www.scopus.com/inward/record.url?scp=85052503109&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-8709-2_8
DO - 10.1007/978-1-4939-8709-2_8
M3 - Chapter
C2 - 30141011
AN - SCOPUS:85052503109
T3 - Methods in Molecular Biology
SP - 119
EP - 137
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
