TY - JOUR
T1 - Determination of acrylamide and glycidamide in rat plasma by reversed-phase high performance liquid chromatography
AU - Barber, David S.
AU - Hunt, Jason
AU - LoPachin, Richard M.
AU - Ehrich, Marion
N1 - Funding Information:
This work was supported by the Virginia-Maryland Regional College of Veterinary Medicine and NIEHS grant ES03830 awarded to Dr R.M. LoPachin.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - Acrylamide is a widely used monomer that produces peripheral neuropathy. It is metabolized to the epoxide, glycidamide, which is also considered to be neurotoxic. A new reversed-phase high-performance liquid chromatography (HPLC) method is described that permits simultaneous determination of acrylamide and glycidamide in rat plasma. Samples were deproteinized with acetonitrile and chromatography was performed using isocratic elution and UV absorption detection. The limits of detection for acrylamide and glycidamide were 0.05 and 0.25 μg/ml in plasma, respectively, and recovery of both analytes was greater than 90%. The assay was linear from 0.1 to 100 μg/ml for acrylamide and from 0.5 to 100 μg/ml for glycidamide. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration - time profiles of acrylamide and glycidamide in the plasma of acrylamide-treated rats.
AB - Acrylamide is a widely used monomer that produces peripheral neuropathy. It is metabolized to the epoxide, glycidamide, which is also considered to be neurotoxic. A new reversed-phase high-performance liquid chromatography (HPLC) method is described that permits simultaneous determination of acrylamide and glycidamide in rat plasma. Samples were deproteinized with acetonitrile and chromatography was performed using isocratic elution and UV absorption detection. The limits of detection for acrylamide and glycidamide were 0.05 and 0.25 μg/ml in plasma, respectively, and recovery of both analytes was greater than 90%. The assay was linear from 0.1 to 100 μg/ml for acrylamide and from 0.5 to 100 μg/ml for glycidamide. Variation over the range of the standard curve was less than 15%. The method was used to determine the concentration - time profiles of acrylamide and glycidamide in the plasma of acrylamide-treated rats.
KW - Acrylamide
KW - Glycidamide
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U2 - 10.1016/S0378-4347(01)00191-8
DO - 10.1016/S0378-4347(01)00191-8
M3 - Article
C2 - 11486839
AN - SCOPUS:0035879918
SN - 1387-2273
VL - 758
SP - 289
EP - 293
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -