Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon; Harry Malech; Graeme Milligan; Cecilia Unson; Richard Kahn; Paul Goldsmith; Allen Spiegel

doi:10.1016/0014-5793(86)81141-3

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Judith Falloon
, Harry Malech
, Graeme Milligan
, Cecilia Unson
, Richard Kahn
, Paul Goldsmith
, Allen Spiegel

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

Original language	English (US)
Pages (from-to)	352-356
Number of pages	5
Journal	FEBS Letters
Volume	209
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)81141-3
State	Published - Dec 15 1986
Externally published	Yes

Keywords

GTP-binding protein
Phosphoinositide breakdown
Signal transduction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(86)81141-3

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title = "Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides",

abstract = "Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.",

keywords = "GTP-binding protein, Phosphoinositide breakdown, Signal transduction",

author = "Judith Falloon and Harry Malech and Graeme Milligan and Cecilia Unson and Richard Kahn and Paul Goldsmith and Allen Spiegel",

year = "1986",

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doi = "10.1016/0014-5793(86)81141-3",

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T1 - Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

AU - Falloon, Judith

AU - Malech, Harry

AU - Milligan, Graeme

AU - Unson, Cecilia

AU - Kahn, Richard

AU - Goldsmith, Paul

AU - Spiegel, Allen

PY - 1986/12/15

Y1 - 1986/12/15

N2 - Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

AB - Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

KW - GTP-binding protein

KW - Phosphoinositide breakdown

KW - Signal transduction

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UR - https://www.scopus.com/pages/publications/0022864104#tab=citedBy

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DO - 10.1016/0014-5793(86)81141-3

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AN - SCOPUS:0022864104

SN - 0014-5793

VL - 209

SP - 352

EP - 356

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

Abstract

Keywords

ASJC Scopus subject areas

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