Detection of seven species of pathogenic leptospires by PCR using two sets of primers

C. Gravekamp; H. Van de Kemp; M. Franzen; D. Carrington; G. J. Schoone; G. J.J.M. Van Eys; C. O.R. Everard; R. A. Hartskeerl; W. J. Terpstra

doi:10.1099/00221287-139-8-1691

Detection of seven species of pathogenic leptospires by PCR using two sets of primers

C. Gravekamp, H. Van de Kemp, M. Franzen, D. Carrington, G. J. Schoone, G. J.J.M. Van Eys, C. O.R. Everard, R. A. Hartskeerl, W. J. Terpstra

Research output: Contribution to journal › Article › peer-review

249 Scopus citations

Abstract

Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii, L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.

Original language	English (US)
Pages (from-to)	1691-1700
Number of pages	10
Journal	Journal of General Microbiology
Volume	139
Issue number	8
DOIs	https://doi.org/10.1099/00221287-139-8-1691
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Microbiology

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title = "Detection of seven species of pathogenic leptospires by PCR using two sets of primers",

abstract = "Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii, L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.",

author = "C. Gravekamp and {Van de Kemp}, H. and M. Franzen and D. Carrington and Schoone, {G. J.} and {Van Eys}, {G. J.J.M.} and Everard, {C. O.R.} and Hartskeerl, {R. A.} and Terpstra, {W. J.}",

year = "1993",

doi = "10.1099/00221287-139-8-1691",

language = "English (US)",

volume = "139",

pages = "1691--1700",

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T1 - Detection of seven species of pathogenic leptospires by PCR using two sets of primers

AU - Gravekamp, C.

AU - Van de Kemp, H.

AU - Franzen, M.

AU - Carrington, D.

AU - Schoone, G. J.

AU - Van Eys, G. J.J.M.

AU - Everard, C. O.R.

AU - Hartskeerl, R. A.

AU - Terpstra, W. J.

PY - 1993

Y1 - 1993

N2 - Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii, L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.

AB - Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii, L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.

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Detection of seven species of pathogenic leptospires by PCR using two sets of primers

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