Design and application of 2-D DGGE-based gene mutational scanning tests

Nathalie J. Van Orsouw; Jan Vijg

doi:10.1016/S1050-3862(98)00028-X

Design and application of 2-D DGGE-based gene mutational scanning tests

Nathalie J. Van Orsouw
, Jan Vijg

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.

Original language	English (US)
Pages (from-to)	205-213
Number of pages	9
Journal	Genetic Analysis - Biomolecular Engineering
Volume	14
Issue number	5-6
DOIs	https://doi.org/10.1016/S1050-3862(98)00028-X
State	Published - Feb 1999
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

2-D DGGE
Gene mutational scanning tests
PCR

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Genetics

Access to Document

10.1016/S1050-3862(98)00028-X

Cite this

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title = "Design and application of 2-D DGGE-based gene mutational scanning tests",

abstract = "Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.",

keywords = "2-D DGGE, Gene mutational scanning tests, PCR",

author = "\{Van Orsouw\}, \{Nathalie J.\} and Jan Vijg",

note = "Funding Information: We thank Dr C. Eng for critically reading the manuscript, and we would like to acknowledge Drs C. Eng and I. Sigalas and R.K. Dhanda, R.-D. Rines and W. Smith for contributing to the work described in this paper. We are also grateful to Dr L. Lerman for his helpful suggestions during the development of TDGS. This research was supported by the Massachusetts Department of Public Health (3408799DO47), the Harvard Nathan Shock Center of Excellence in the Basic Biology of Aging (1P30AG13314-01) and research funds from Beltronics Inc., Newton, MA.",

year = "1999",

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doi = "10.1016/S1050-3862(98)00028-X",

language = "English (US)",

volume = "14",

pages = "205--213",

journal = "Genetic Analysis - Biomolecular Engineering",

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T1 - Design and application of 2-D DGGE-based gene mutational scanning tests

AU - Van Orsouw, Nathalie J.

AU - Vijg, Jan

N1 - Funding Information: We thank Dr C. Eng for critically reading the manuscript, and we would like to acknowledge Drs C. Eng and I. Sigalas and R.K. Dhanda, R.-D. Rines and W. Smith for contributing to the work described in this paper. We are also grateful to Dr L. Lerman for his helpful suggestions during the development of TDGS. This research was supported by the Massachusetts Department of Public Health (3408799DO47), the Harvard Nathan Shock Center of Excellence in the Basic Biology of Aging (1P30AG13314-01) and research funds from Beltronics Inc., Newton, MA.

PY - 1999/2

Y1 - 1999/2

N2 - Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.

AB - Currently, there is a need for practical, accurate and cost-efficient tests to comprehensively scan human genes for disease-related DNA sequence variation. Two-dimensional gene scanning (TDGS) is a parallel mutation detection system, based on a combination of extensive multiplex PCR amplification ('PCR megaplex') and two-dimensional (2-D) DNA electrophoresis. The latter comprises a size separation step followed by denaturing gradient gel electrophoresis (DGGE), and allows single base pair changes to be distinguished among multiple DNA fragments in parallel. Here, we describe the rapid design of TDGS tests and its application to mutation identification in several large human cancer genes.

KW - 2-D DGGE

KW - Gene mutational scanning tests

KW - PCR

UR - https://www.scopus.com/pages/publications/0032971221

UR - https://www.scopus.com/pages/publications/0032971221#tab=citedBy

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DO - 10.1016/S1050-3862(98)00028-X

M3 - Article

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AN - SCOPUS:0032971221

SN - 1050-3862

VL - 14

SP - 205

EP - 213

JO - Genetic Analysis - Biomolecular Engineering

JF - Genetic Analysis - Biomolecular Engineering

IS - 5-6

ER -

Design and application of 2-D DGGE-based gene mutational scanning tests

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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