Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein

Olena S. Oliinyk, Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Toboada, Huaxin Sheng, Junjie Yao, Vladislav V. Verkhusha

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Applying rational design, we developed 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano. miRFP718nano efficiently binds endogenous biliverdin chromophore and brightly fluoresces in mammalian cells and tissues. miRFP718nano has maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, allowing deep-penetrating off-peak fluorescence imaging in vivo. The miRFP718nano structure reveals the molecular basis of its red shift. We demonstrate superiority of miRFP718nano-enabled SWIR imaging over NIR-I imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland and NF-kB activity in a mouse model of liver inflammation.

Original languageEnglish (US)
Pages (from-to)70-74
Number of pages5
JournalNature Methods
Volume20
Issue number1
DOIs
StatePublished - Jan 2023

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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