Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia; Ann Kindt; Hernan Bermudez; Alejandro Llanos-Cuentas; Simonne De Doncker; Jorge Arevalo; Kelly Wilber Quispe Tintaya; Jean Claude Dujardin

doi:10.1128/JCM.42.5.2294-2297.2004

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia, Ann Kindt, Hernan Bermudez, Alejandro Llanos-Cuentas, Simonne De Doncker, Jorge Arevalo, Kelly Wilber Quispe Tintaya, Jean Claude Dujardin

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168 Scopus citations

Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Original language	English (US)
Pages (from-to)	2294-2297
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	5
DOIs	https://doi.org/10.1128/JCM.42.5.2294-2297.2004
State	Published - May 2004
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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Garcia, L., Kindt, A., Bermudez, H., Llanos-Cuentas, A., De Doncker, S., Arevalo, J., Tintaya, K. W. Q., & Dujardin, J. C. (2004). Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes. Journal of Clinical Microbiology, 42(5), 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004

Garcia, L, Kindt, A, Bermudez, H, Llanos-Cuentas, A, De Doncker, S, Arevalo, J, Tintaya, KWQ & Dujardin, JC 2004, 'Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes', Journal of Clinical Microbiology, vol. 42, no. 5, pp. 2294-2297. https://doi.org/10.1128/JCM.42.5.2294-2297.2004

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AU - Tintaya, Kelly Wilber Quispe

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Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Abstract

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