Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit

O. S. Oliinyk; A. A. Kaberniuk; T. A. Redchuk; N. V. Korotkevich; A. J. Labyntsev; S. I. Romanyuk; D. V. Kolibo; S. V. Komisarenko

Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit

O. S. Oliinyk, A. A. Kaberniuk, T. A. Redchuk, N. V. Korotkevich, A. J. Labyntsev, S. I. Romanyuk, D. V. Kolibo, S. V. Komisarenko

Anatomy & Structural Biology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 ⁷ to 10 ⁹ M ^-1.

Original language	English (US)
Pages (from-to)	68-79
Number of pages	12
Journal	Ukrain'skyi Biokhimichnyi Zhurnal
Volume	81
Issue number	2
State	Published - Jul 7 2009

Keywords

Diphtheria toxin
Library of immunoglobulin genes
Phage display
Scfv
Single chain antibodies

ASJC Scopus subject areas

Biochemistry

Cite this

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title = "Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit",

abstract = "The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 7 to 10 9 M -1.",

keywords = "Diphtheria toxin, Library of immunoglobulin genes, Phage display, Scfv, Single chain antibodies",

author = "Oliinyk, {O. S.} and Kaberniuk, {A. A.} and Redchuk, {T. A.} and Korotkevich, {N. V.} and Labyntsev, {A. J.} and Romanyuk, {S. I.} and Kolibo, {D. V.} and Komisarenko, {S. V.}",

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language = "English (US)",

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pages = "68--79",

journal = "Ukrain'skyi Biokhimichnyi Zhurnal",

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publisher = "Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU)",

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AU - Oliinyk, O. S.

AU - Kaberniuk, A. A.

AU - Redchuk, T. A.

AU - Korotkevich, N. V.

AU - Labyntsev, A. J.

AU - Romanyuk, S. I.

AU - Kolibo, D. V.

AU - Komisarenko, S. V.

PY - 2009/7/7

Y1 - 2009/7/7

N2 - The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 7 to 10 9 M -1.

AB - The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 7 to 10 9 M -1.

KW - Diphtheria toxin

KW - Library of immunoglobulin genes

KW - Phage display

KW - Scfv

KW - Single chain antibodies

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Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit

Abstract

Keywords

ASJC Scopus subject areas

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