Complementation between mutants of CHO cells resistant to a variety of plant lectins

Pamela Stanley; Louis Siminovitch

doi:10.1007/BF01542968

Complementation between mutants of CHO cells resistant to a variety of plant lectins

Pamela Stanley, Louis Siminovitch

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Abstract

Chinese hamster cell mutants resistant to the lectins PHA, WGA, RIC, LCA, and CON A were previously grouped into 8-10 distinct phenotypes on the basis of their unique patterns of lectin resistance and lectin-binding properties. All but one of these classes of lectin-resistant (Lec^R) mutants behave recessively in somatic cell hybrids. One ricin-resistant class ( {Mathematical expression} behaves dominantly. Test for complementation, by measuring the lectin-resistant properties of appropriate hybrids, show that seven distinct complementation groups can be delineated among the phenotypically recessive mutants.

Original language	English (US)
Pages (from-to)	391-405
Number of pages	15
Journal	Somatic Cell Genetics
Volume	3
Issue number	4
DOIs	https://doi.org/10.1007/BF01542968
State	Published - Jul 1 1977
Externally published	Yes

ASJC Scopus subject areas

Genetics

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AU - Siminovitch, Louis

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AB - Chinese hamster cell mutants resistant to the lectins PHA, WGA, RIC, LCA, and CON A were previously grouped into 8-10 distinct phenotypes on the basis of their unique patterns of lectin resistance and lectin-binding properties. All but one of these classes of lectin-resistant (LecR) mutants behave recessively in somatic cell hybrids. One ricin-resistant class ( {Mathematical expression} behaves dominantly. Test for complementation, by measuring the lectin-resistant properties of appropriate hybrids, show that seven distinct complementation groups can be delineated among the phenotypically recessive mutants.

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Complementation between mutants of CHO cells resistant to a variety of plant lectins

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