TY - JOUR
T1 - Cloning of AIP1, a novel protein that associates with the apoptosis- linked gene ALG-2 in a Ca2+-dependent reaction
AU - Vito, Pasquale
AU - Pellegrini, Luca
AU - Guiet, Chantal
AU - D'Adamio, Luciano
PY - 1999/1/15
Y1 - 1999/1/15
N2 - ALG-2 is a 22-kDa calcium-binding protein necessary for cell death induced by different stimuli in 3DO T-cell hyhridoma. 3DO cell clones depleted of ALG-2 protein exhibit normal caspases activation, suggesting that ALG-2 function is required downstream or is independent of caspase proteases activity for apoptosis to occur. Using the yeast two-hybrid screening system, we have isolated and characterized the mouse cDNA encoding for ALG-2 interacting protein 1 (AIP1), a novel protein that interacts with ALG-2. ALG- 2 and AIP1 colocalize in the cytosol and the presence of calcium is an indispensable requisite for their association. Sequence alignment shows that AIP1 is highly similar to BRO1, a yeast protein related to components of the Pkc1p-MAP kinase cascade. Overexpression of a truncated form of AIP1 protects two different cell types from death induced by trophic factors withdrawal; thus, our data indicate that AIP1 cooperates with ALG-2 in executing the calcium-dependent requirements along the cell death pathway.
AB - ALG-2 is a 22-kDa calcium-binding protein necessary for cell death induced by different stimuli in 3DO T-cell hyhridoma. 3DO cell clones depleted of ALG-2 protein exhibit normal caspases activation, suggesting that ALG-2 function is required downstream or is independent of caspase proteases activity for apoptosis to occur. Using the yeast two-hybrid screening system, we have isolated and characterized the mouse cDNA encoding for ALG-2 interacting protein 1 (AIP1), a novel protein that interacts with ALG-2. ALG- 2 and AIP1 colocalize in the cytosol and the presence of calcium is an indispensable requisite for their association. Sequence alignment shows that AIP1 is highly similar to BRO1, a yeast protein related to components of the Pkc1p-MAP kinase cascade. Overexpression of a truncated form of AIP1 protects two different cell types from death induced by trophic factors withdrawal; thus, our data indicate that AIP1 cooperates with ALG-2 in executing the calcium-dependent requirements along the cell death pathway.
UR - http://www.scopus.com/inward/record.url?scp=0033556043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033556043&partnerID=8YFLogxK
U2 - 10.1074/jbc.274.3.1533
DO - 10.1074/jbc.274.3.1533
M3 - Article
C2 - 9880530
AN - SCOPUS:0033556043
SN - 0021-9258
VL - 274
SP - 1533
EP - 1540
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 3
ER -