Clonal growth in vitro by mouse Kupffer cells

Dien Ming Chen; Hsiu San Lin; Philip Stahl; E. Richard Stanley

doi:10.1016/0014-4827(79)90449-X

Clonal growth in vitro by mouse Kupffer cells

Dien Ming Chen, Hsiu San Lin, Philip Stahl, E. Richard Stanley

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Mouse liver Kupffer cells were induced to proliferate and form discrete colonies of mononuclear phagocytes in vitro. These colony-forming cells from the liver are similar to other mononuclear phagocyte colony-forming cells in that they require a colony-stimulating factor present in medium conditioned by L cells for proliferation in vitro. Cells in the colonies were phagocytic and had IgG receptors on the membrane. For this class of colony-forming cells, the D₀ value to gamma irradiation in vitro was 108 rads.

Original language	English (US)
Pages (from-to)	103-109
Number of pages	7
Journal	Experimental Cell Research
Volume	121
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(79)90449-X
State	Published - Jun 1979

ASJC Scopus subject areas

Cell Biology

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title = "Clonal growth in vitro by mouse Kupffer cells",

abstract = "Mouse liver Kupffer cells were induced to proliferate and form discrete colonies of mononuclear phagocytes in vitro. These colony-forming cells from the liver are similar to other mononuclear phagocyte colony-forming cells in that they require a colony-stimulating factor present in medium conditioned by L cells for proliferation in vitro. Cells in the colonies were phagocytic and had IgG receptors on the membrane. For this class of colony-forming cells, the D0 value to gamma irradiation in vitro was 108 rads.",

author = "Chen, {Dien Ming} and Lin, {Hsiu San} and Philip Stahl and Stanley, {E. Richard}",

note = "Funding Information: The authors wish to express their appreciation to Jill Miller for her technical assistance. This investigation was SuDDorted by Grant Number SPOlCA13053-$5, and CA-12858, awarded by the NCI, and HL-19746-02 and GM-21096. awarded bv the National Heart, Lung and Blood Institute, DHEW. H-s. L. is a recipient of Research Career Development Award CA-70730-04 from the NCI.",

year = "1979",

month = jun,

doi = "10.1016/0014-4827(79)90449-X",

language = "English (US)",

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journal = "Experimental Cell Research",

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T1 - Clonal growth in vitro by mouse Kupffer cells

AU - Chen, Dien Ming

AU - Lin, Hsiu San

AU - Stahl, Philip

AU - Stanley, E. Richard

N1 - Funding Information: The authors wish to express their appreciation to Jill Miller for her technical assistance. This investigation was SuDDorted by Grant Number SPOlCA13053-$5, and CA-12858, awarded by the NCI, and HL-19746-02 and GM-21096. awarded bv the National Heart, Lung and Blood Institute, DHEW. H-s. L. is a recipient of Research Career Development Award CA-70730-04 from the NCI.

PY - 1979/6

Y1 - 1979/6

N2 - Mouse liver Kupffer cells were induced to proliferate and form discrete colonies of mononuclear phagocytes in vitro. These colony-forming cells from the liver are similar to other mononuclear phagocyte colony-forming cells in that they require a colony-stimulating factor present in medium conditioned by L cells for proliferation in vitro. Cells in the colonies were phagocytic and had IgG receptors on the membrane. For this class of colony-forming cells, the D0 value to gamma irradiation in vitro was 108 rads.

AB - Mouse liver Kupffer cells were induced to proliferate and form discrete colonies of mononuclear phagocytes in vitro. These colony-forming cells from the liver are similar to other mononuclear phagocyte colony-forming cells in that they require a colony-stimulating factor present in medium conditioned by L cells for proliferation in vitro. Cells in the colonies were phagocytic and had IgG receptors on the membrane. For this class of colony-forming cells, the D0 value to gamma irradiation in vitro was 108 rads.

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Clonal growth in vitro by mouse Kupffer cells

Abstract

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