TY - CHAP
T1 - Chitosan–Stem Cell Interactions
AU - Nanduri, Lalitha Sarad Yamini
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2021
Y1 - 2021
N2 - Chitosan, the deacetylated form of chitin, has been extensively used for tissue engineering in the form of hydrogel, scaffolds, microparticles, nanoparticles, and nanofibers. To develop composite constructs with targeted tissue regeneration, chitosan is often combined with hydroxyapatite, collagen, gelatin, hyaluronic acid, silk fibroin, etc. biomaterials. In addition to this, chitosan is often modified with varied physico-chemical properties. For regenerative application, to improve the efficacy of constructs, chitosan in various forms is combined with various stem cells, among which mesenchymal stem cells (MSCs) have been studied widely. In this review we focused on the studies that exclusively used chitosan in combination with multipotent adipose and bone marrow-derived MSCs and pluripotent-induced pluripotent stem cells (iPSCs). When cultured on chitosan, stem cells displayed greater affinity in terms of cellular adhesion, proliferation, and differentiation into functional cell types both in vitro albeit different potential. When transplanted in vivo, stem cell-laden chitosan constructs showed greater integrity into the host system, differentiated into targeted cells, and demonstrated improved repair of the damaged tissue. These studies provide great insight into the current and future potential of chitosan for regenerative applications.
AB - Chitosan, the deacetylated form of chitin, has been extensively used for tissue engineering in the form of hydrogel, scaffolds, microparticles, nanoparticles, and nanofibers. To develop composite constructs with targeted tissue regeneration, chitosan is often combined with hydroxyapatite, collagen, gelatin, hyaluronic acid, silk fibroin, etc. biomaterials. In addition to this, chitosan is often modified with varied physico-chemical properties. For regenerative application, to improve the efficacy of constructs, chitosan in various forms is combined with various stem cells, among which mesenchymal stem cells (MSCs) have been studied widely. In this review we focused on the studies that exclusively used chitosan in combination with multipotent adipose and bone marrow-derived MSCs and pluripotent-induced pluripotent stem cells (iPSCs). When cultured on chitosan, stem cells displayed greater affinity in terms of cellular adhesion, proliferation, and differentiation into functional cell types both in vitro albeit different potential. When transplanted in vivo, stem cell-laden chitosan constructs showed greater integrity into the host system, differentiated into targeted cells, and demonstrated improved repair of the damaged tissue. These studies provide great insight into the current and future potential of chitosan for regenerative applications.
KW - Cell-laden constructs
KW - Chitosan–stem cell interaction
KW - Exosomes
KW - Stem cells
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U2 - 10.1007/12_2021_83
DO - 10.1007/12_2021_83
M3 - Chapter
AN - SCOPUS:85116464161
T3 - Advances in Polymer Science
SP - 343
EP - 359
BT - Advances in Polymer Science
PB - Springer Science and Business Media Deutschland GmbH
ER -