Cell-type specific expression of p11 controls cocaine reward

Margarita Arango-Lievano, Justin T. Schwarz, Mary Vernov, Matthew B. Wilkinson, Kathryn Bradbury, Akira Feliz, Roberta Marongiu, Yaroslav Gelfand, Jennifer Warner-Schmidt, Eric J. Nestler, Paul Greengard, Scott J. Russo, Michael G. Kaplitt

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Background: The high rate of comorbidity between depression and cocaine addiction suggests shared molecular mechanisms and anatomical pathways. Limbic structures, such as the nucleus accumbens (NAc), play a crucial role in both disorders, yet how different cell types within these structures contribute to the pathogenesis remains elusive. Downregulation of p11 (S100A10), specifically in the NAc, elicits depressive-like behaviors in mice, but its role in drug addiction is unknown.Methods: We combined mouse genetics and viral strategies to determine how the titration of p11 levels within the entire NAc affects the rewarding actions of cocaine on behavior (six to eight mice per group) and molecular correlates (three experiments, five to eight mice per group). Finally, the manipulation of p11 expression in distinct NAc dopaminoceptive neuronal subsets distinguished cell-type specific effects of p11 on cocaine reward (five to eight mice per group). Results: We demonstrated that p11 knockout mice have enhanced cocaine conditioned place preference, which is reproduced by the focal downregulation of p11 in the NAc of wild-type mice. In wild-type mice, cocaine reduced p11 expression in the NAc, while p11 overexpression exclusively in the NAc reduced cocaine conditioned place preference. Finally, we identified dopamine receptor-1 expressing medium spiny neurons as key mediators of the effects of p11 on cocaine reward. Conclusions: Our data provide evidence that disruption of p11 homeostasis in the NAc, particularly in dopamine receptor-1 expressing medium spiny neurons, may underlie pathophysiological mechanisms of cocaine rewarding action. Treatments to counter maladaptation of p11 levels may provide novel therapeutic opportunities for cocaine addiction.

Original languageEnglish (US)
Pages (from-to)794-801
Number of pages8
JournalBiological Psychiatry
Issue number10
StatePublished - 2014
Externally publishedYes


  • Cocaine reward
  • Conditional knock down
  • Nucleus accumbens
  • P11 (s100a10)
  • Striatonigral pathway
  • Striatopallidal pathway

ASJC Scopus subject areas

  • Biological Psychiatry


Dive into the research topics of 'Cell-type specific expression of p11 controls cocaine reward'. Together they form a unique fingerprint.

Cite this