cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells

M. B. Ladner; G. A. Martin; J. A. Noble; V. P. Wittman; M. K. Warren; M. McGrogan; E. R. Stanley

doi:10.1073/pnas.85.18.6706

cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells

M. B. Ladner, G. A. Martin, J. A. Noble, V. P. Wittman, M. K. Warren, M. McGrogan, E. R. Stanley

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Abstract

A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.

Original language	English (US)
Pages (from-to)	6706-6710
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	18
DOIs	https://doi.org/10.1073/pnas.85.18.6706
State	Published - 1988
Externally published	Yes

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title = "cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells",

abstract = "A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.",

author = "Ladner, {M. B.} and Martin, {G. A.} and Noble, {J. A.} and Wittman, {V. P.} and Warren, {M. K.} and M. McGrogan and Stanley, {E. R.}",

year = "1988",

doi = "10.1073/pnas.85.18.6706",

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T1 - cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells

AU - Ladner, M. B.

AU - Martin, G. A.

AU - Noble, J. A.

AU - Wittman, V. P.

AU - Warren, M. K.

AU - McGrogan, M.

AU - Stanley, E. R.

PY - 1988

Y1 - 1988

N2 - A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.

AB - A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.

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JF - Proceedings of the National Academy of Sciences of the United States of America

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cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells

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