@article{61e011850c7c43b7a7fcefa8c3aa031b,
title = "CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes",
abstract = "Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f+ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.",
keywords = "A1 reactive astrocytes, CD49f, FACS purification, astrocytes, induced pluripotent stem cells, neurodegeneration, neurotoxicity",
author = "Lilianne Barbar and Tanya Jain and Matthew Zimmer and Ilya Kruglikov and Sadick, {Jessica S.} and Minghui Wang and Kriti Kalpana and Rose, {Indigo V.L.} and Burstein, {Suzanne R.} and Tomasz Rusielewicz and Madhura Nijsure and Guttenplan, {Kevin A.} and {di Domenico}, Angelique and Gist Croft and Bin Zhang and Hiroko Nobuta and H{\'e}bert, {Jean M.} and Liddelow, {Shane A.} and Valentina Fossati",
note = "Funding Information: This work was supported by the National Stem Cell Foundation (NSCF), the New York Stem Cell Foundation Research Institute (NYSCF) (including all experiments involving fetal tissues), National Institute of Neurological Disorders and Stroke (NINDS) grant 1R21NS111186 and NYSTEM grant DOH01-STEM5-2016-00114 (to V.F.), National Institute on Aging (NIA) grants U01AG046170 and RF1AG057440 (to B.Z.), the NYU School of Medicine, anonymous donors, the Blas Frangione Foundation, and the Cure AD Fund (to S.A.L.). Schematics were created using BioRender.com. We thank Jim Goldman for the O4 antibody; Davide Marotta and Anahita Sharma for assistance with FACS analysis and organoid sectioning, respectively; Daniel Paull and the NYSCF Global Stem Cell Array team for iPSC line derivation; Edward Stopa, Terra D. Velilla, and the Brain Tissue Resource Center for providing primary human AD brain samples; Raeka Aiyar for manuscript editing; and Frederick Monsma, Maria Sapar, and all members of the lab for critical feedback. The bulk RNA-seq and scRNA-seq data are available from the Synapse open source platform (Synapse: https://www.synapse.org/#!Synapse:syn21861229). Conceptualization, V.F.; Validation, L.B.; Formal Analysis, L.B. J.S. S.A.L. M.W. and B.Z.; Investigation, L.B. T.J. I.K. M.Z. K.K. I.V.L.R. K.A.G. M.N. and A.d.D.; Resources, H.N. and J.M.H.; Writing – Original Draft, V.F. and L.B.; Writing – Review & Editing, V.F. L.B. S.A.L. and all authors; Visualization, L.B. I.K. J.S. I.V.L.R. M.Z. and M.W.; Supervision, V.F. S.A.L. S.B. T.R. and G.C.; Project Administration, V.F.; Funding Acquisition, V.F. and S.A.L. S.A.L. is an academic founder of AstronauTx Ltd. NYSCF. U.S. patent pending. Funding Information: This work was supported by the National Stem Cell Foundation (NSCF), the New York Stem Cell Foundation Research Institute (NYSCF) (including all experiments involving fetal tissues), National Institute of Neurological Disorders and Stroke (NINDS) grant 1R21NS111186 and NYSTEM grant DOH01-STEM5-2016-00114 (to V.F.), National Institute on Aging (NIA) grants U01AG046170 and RF1AG057440 (to B.Z.), the NYU School of Medicine , anonymous donors, the Blas Frangione Foundation , and the Cure AD Fund (to S.A.L.). Schematics were created using BioRender.com. We thank Jim Goldman for the O4 antibody; Davide Marotta and Anahita Sharma for assistance with FACS analysis and organoid sectioning, respectively; Daniel Paull and the NYSCF Global Stem Cell Array team for iPSC line derivation; Edward Stopa, Terra D. Velilla, and the Brain Tissue Resource Center for providing primary human AD brain samples; Raeka Aiyar for manuscript editing; and Frederick Monsma, Maria Sapar, and all members of the lab for critical feedback. The bulk RNA-seq and scRNA-seq data are available from the Synapse open source platform (Synapse: https://www.synapse.org/#!Synapse:syn21861229 ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = aug,
day = "5",
doi = "10.1016/j.neuron.2020.05.014",
language = "English (US)",
volume = "107",
pages = "436--453.e12",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}