CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Lilianne Barbar; Tanya Jain; Matthew Zimmer; Ilya Kruglikov; Jessica S. Sadick; Minghui Wang; Kriti Kalpana; Indigo V.L. Rose; Suzanne R. Burstein; Tomasz Rusielewicz; Madhura Nijsure; Kevin A. Guttenplan; Angelique di Domenico; Gist Croft; Bin Zhang; Hiroko Nobuta; Jean M. Hébert; Shane A. Liddelow; Valentina Fossati

doi:10.1016/j.neuron.2020.05.014

CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Lilianne Barbar, Tanya Jain, Matthew Zimmer, Ilya Kruglikov, Jessica S. Sadick, Minghui Wang, Kriti Kalpana, Indigo V.L. Rose, Suzanne R. Burstein, Tomasz Rusielewicz, Madhura Nijsure, Kevin A. Guttenplan, Angelique di Domenico, Gist Croft, Bin Zhang, Hiroko Nobuta, Jean M. Hébert, Shane A. Liddelow, Valentina Fossati

Neuroscience

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f⁺ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.

Original language	English (US)
Pages (from-to)	436-453.e12
Journal	Neuron
Volume	107
Issue number	3
DOIs	https://doi.org/10.1016/j.neuron.2020.05.014
State	Published - Aug 5 2020

Keywords

A1 reactive astrocytes
CD49f
FACS purification
astrocytes
induced pluripotent stem cells
neurodegeneration
neurotoxicity

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2020.05.014

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Barbar, L., Jain, T., Zimmer, M., Kruglikov, I., Sadick, J. S., Wang, M., Kalpana, K., Rose, I. V. L., Burstein, S. R., Rusielewicz, T., Nijsure, M., Guttenplan, K. A., di Domenico, A., Croft, G., Zhang, B., Nobuta, H., Hébert, J. M., Liddelow, S. A., & Fossati, V. (2020). CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes. Neuron, 107(3), 436-453.e12. https://doi.org/10.1016/j.neuron.2020.05.014

Barbar, L, Jain, T, Zimmer, M, Kruglikov, I, Sadick, JS, Wang, M, Kalpana, K, Rose, IVL, Burstein, SR, Rusielewicz, T, Nijsure, M, Guttenplan, KA, di Domenico, A, Croft, G, Zhang, B, Nobuta, H, Hébert, JM, Liddelow, SA & Fossati, V 2020, 'CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes', Neuron, vol. 107, no. 3, pp. 436-453.e12. https://doi.org/10.1016/j.neuron.2020.05.014

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abstract = "Barbar et al. identify CD49f as a novel surface marker expressed by human astrocytes that can purify hiPSC-astrocytes and primary fetal astrocytes. CD49f+ hiPSC-astrocytes respond to pro-inflammatory stimuli and become A1 reactive astrocytes, which are dysfunctional and secrete neurotoxic factors that induce apoptosis in human and rodent neurons.",

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AU - Zimmer, Matthew

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AU - Sadick, Jessica S.

AU - Wang, Minghui

AU - Kalpana, Kriti

AU - Rose, Indigo V.L.

AU - Burstein, Suzanne R.

AU - Rusielewicz, Tomasz

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AU - Guttenplan, Kevin A.

AU - di Domenico, Angelique

AU - Croft, Gist

AU - Zhang, Bin

AU - Nobuta, Hiroko

AU - Hébert, Jean M.

AU - Liddelow, Shane A.

AU - Fossati, Valentina

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CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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