Calculating glucose fluxes during meal tolerance test: A new computational approach

Roman Hovorka, Harsha Jayatillake, Eduard Rogatsky, Vlad Tomuta, Tomas Hovorka, Daniel T. Stein

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A new calculation method is proposed to quantify the endogenous glucose production (EGP), the glucose appearance rate due to meal ingestion (R a meal), and the glucose disposal (Rd) during a three-tracer study design. The method utilizes the maximum likelihood theory combined with a regularization method to achieve a theoretically coherent computational framework. The method uses the two-compartment formulation of the glucose kinetics. Instead of assuming smoothness of unlabeled and labeled glucose concentrations, the method assumes that the EGP, the Ra meal, and the fractional glucose clearance are smooth, increasing plausibility of their individual estimates. The method avoids transformation of the measurement errors, which may skew the estimates of the EGP, Ra meal, and R d with the traditional approach. Finally, the sequential nature of the calculations is replaced by calculating the EGP, Ra meal, and Rd in "one go" to avoid the propagation of the errors from the EGP and Ra meal into Rd. An example study is shown demonstrating the utility of the approach. A better performance of the new method is demonstrated in a simulation study.

Original languageEnglish (US)
Pages (from-to)E610-E619
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Issue number2
StatePublished - Aug 2007


  • Endogenous glucose production
  • Glucose kinetics
  • Glucose tracer
  • Gut absorption
  • Mathematical modeling

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)


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