Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni

Irving Nachamkin; Ruth Hogue Angeletti; Michael B. Prystowsky

doi:10.1111/j.1574-6968.1988.tb02987.x

Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni

Irving Nachamkin
, Ruth Hogue Angeletti
, Michael B. Prystowsky

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.

Original language	English (US)
Pages (from-to)	149-152
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	51
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1988.tb02987.x
State	Published - Jun 15 1988
Externally published	Yes

Keywords

(Amino acid sequence)
(HPLC-gel permeation)
Campylobacter jejuni
Flagellin

ASJC Scopus subject areas

General Medicine

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10.1111/j.1574-6968.1988.tb02987.x

Cite this

@article{d9108f99419a4b7da0a2fc8c0a37171d,

title = "Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni",

abstract = "We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.",

keywords = "(Amino acid sequence), (HPLC-gel permeation), Campylobacter jejuni, Flagellin",

author = "Irving Nachamkin and Angeletti, \{Ruth Hogue\} and Prystowsky, \{Michael B.\}",

note = "Funding Information: We thank Xiao-He Yang for excellent technical assistance. Supported in part by BRSG SO7-RR-05415-26 from the National Institutes of Health.",

year = "1988",

month = jun,

day = "15",

doi = "10.1111/j.1574-6968.1988.tb02987.x",

language = "English (US)",

volume = "51",

pages = "149--152",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "2-3",

}

TY - JOUR

T1 - Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni

AU - Nachamkin, Irving

AU - Angeletti, Ruth Hogue

AU - Prystowsky, Michael B.

N1 - Funding Information: We thank Xiao-He Yang for excellent technical assistance. Supported in part by BRSG SO7-RR-05415-26 from the National Institutes of Health.

PY - 1988/6/15

Y1 - 1988/6/15

N2 - We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.

AB - We describe a simplified method for obtaining highly purified flagellin, suitable for biochemical analysis using HPLC-gel permeation. Amino acid composition and N-terminal sequence analyses were performed on flagellins from serologically distinct isolates. The amino acid composition of flagellin from 10 strains was very similar. The N-terminal amino acid sequence is highly conserved. Significant sequence homology was found with flagellin of Bacillus subtilis.

KW - (Amino acid sequence)

KW - (HPLC-gel permeation)

KW - Campylobacter jejuni

KW - Flagellin

UR - https://www.scopus.com/pages/publications/0023924942

UR - https://www.scopus.com/pages/publications/0023924942#tab=citedBy

U2 - 10.1111/j.1574-6968.1988.tb02987.x

DO - 10.1111/j.1574-6968.1988.tb02987.x

M3 - Article

AN - SCOPUS:0023924942

SN - 0378-1097

VL - 51

SP - 149

EP - 152

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Biochemical similarity among serologically distinct flagellins of Campylobacter jejuni

Abstract

Keywords

ASJC Scopus subject areas

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