Abstract
Previous studies show that expression of heme oxygenase-1 (HO-1) in endothelial cells results in decreased cyclooxygenase expression and prostaglandin (PG) levels through limiting heme availability. Regulation of PGs, important inflammatory mediators, may contribute to the anti-inflammatory potential of HO-1. Here we examine the effects of HO-1 expression on PG clearance via the prostaglandin transporter (PGT). Endothelial cells expressing human HO-1 via retroviral transfer exhibit ∼7-fold higher levels of PGT RNA and equivalently elevated uptake of [ 3H]PGE 2. The pattern and extent of uptake and the substrate inhibitory constants of PGE 2, PGF 2α, and thromboxane B 2 are similar to those of cloned PGT. Treatment of cells with stannous chloride, an inducer of HO-1, results in increased expression of PGT while incubation of cells expressing human HO-1 with stannic mesophorphyrin, a substrate inhibitor of HO-1, decreases PG uptake. Therefore, PG clearance via PGT may contribute to the cellular regulation of PG levels by HO-1.
Original language | English (US) |
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Pages (from-to) | 1299-1305 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 323 |
Issue number | 4 |
DOIs | |
State | Published - Oct 29 2004 |
Keywords
- Endothelial cells
- Heme oxygenase-1
- Inflammation
- Prostaglandin clearance
- Prostaglandin transporter
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology