TY - JOUR
T1 - Application of cell-based biological bioassays for health risk assessment of PM2.5 exposure in three megacities, China
AU - Chen, Shen
AU - Li, Daochuan
AU - Wu, Xiaonen
AU - Chen, Liping
AU - Zhang, Bin
AU - Tan, Yafei
AU - Yu, Dianke
AU - Niu, Yong
AU - Duan, Huawei
AU - Li, Qiong
AU - Chen, Rui
AU - Aschner, Michael
AU - Zheng, Yuxin
AU - Chen, Wen
N1 - Funding Information:
We thank Dr. Mai, Bixuan, Dr. Chunying Chen, and Prof. Yu, Jianzhen for the kind help in analysis of PM2.5 compositions. This work was supported by the Major Research Plan Integration Project of National Natural Science Foundation of China ( 91943301 ), Guangdong Provincial Natural Science Foundation Team Project ( 2018B030312005 ), National Institute of Environmental Health Sciences (NIEHS) of USA ( R01 ES10563 , R01 ES07331 and R01 ES020852 ).
Publisher Copyright:
© 2020 The Authors
PY - 2020/6
Y1 - 2020/6
N2 - The determination of PM2.5-induced biological response is essential for understanding the adverse health risk associated with PM2.5 exposure. In this study, we conducted cell-based bioassays to measure the toxic effects of PM2.5 exposure, including cytotoxicity, oxidative stress, genotoxicity and inflammatory response. The concentration-response relationship was analyzed by benchmark dose (BMD) modeling and the BMDL10 was used to estimate the biological potency of PM2.5 exposure. PM2.5 samples were collected from three typical megacities of China (Beijing, BJ; Wuhan, WH; Guangzhou, GZ) in typical seasons (winter and summer). The total PM, water-soluble fractions (WSF), and organic extracts (OE) were prepared and subjected to examination of toxic effects. The biological potencies for cytotoxicity, oxidative stress and genotoxicity were generally higher in winter samples, while the inflammatory potency of PM2.5 was higher in summer samples. The relative health risk (RHR) was determined by integration of the biological potencies and the cumulative exposure level, and the ranks of RHR were BJ-W > WH-W > BJ-S > WH-S > GZ-W > GZ-S. Notably, we note that different PM2.5 compositions were associated with distinct biological effects, and the health effects distribution of PM2.5 varied in regions and seasons. These findings demonstrate that the approach of integrated cell-based bioassays could be used for the evaluation of health effects of PM2.5 exposure.
AB - The determination of PM2.5-induced biological response is essential for understanding the adverse health risk associated with PM2.5 exposure. In this study, we conducted cell-based bioassays to measure the toxic effects of PM2.5 exposure, including cytotoxicity, oxidative stress, genotoxicity and inflammatory response. The concentration-response relationship was analyzed by benchmark dose (BMD) modeling and the BMDL10 was used to estimate the biological potency of PM2.5 exposure. PM2.5 samples were collected from three typical megacities of China (Beijing, BJ; Wuhan, WH; Guangzhou, GZ) in typical seasons (winter and summer). The total PM, water-soluble fractions (WSF), and organic extracts (OE) were prepared and subjected to examination of toxic effects. The biological potencies for cytotoxicity, oxidative stress and genotoxicity were generally higher in winter samples, while the inflammatory potency of PM2.5 was higher in summer samples. The relative health risk (RHR) was determined by integration of the biological potencies and the cumulative exposure level, and the ranks of RHR were BJ-W > WH-W > BJ-S > WH-S > GZ-W > GZ-S. Notably, we note that different PM2.5 compositions were associated with distinct biological effects, and the health effects distribution of PM2.5 varied in regions and seasons. These findings demonstrate that the approach of integrated cell-based bioassays could be used for the evaluation of health effects of PM2.5 exposure.
KW - Biological potency
KW - Cytotoxicity
KW - Genotoxicity
KW - Health risk assessment
KW - Inflammatory response
KW - Oxidative stress
KW - PM
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U2 - 10.1016/j.envint.2020.105703
DO - 10.1016/j.envint.2020.105703
M3 - Article
C2 - 32259755
AN - SCOPUS:85082872812
SN - 0160-4120
VL - 139
JO - Environment international
JF - Environment international
M1 - 105703
ER -
