Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

Jon W. Gordon; Joan Uehlinger; Nooshine Dayani; Beth E. Talansky; Mildred Gordon; Greg S. Rudomen; Paul E. Neumann

doi:10.1016/0012-1606(90)90259-L

Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

Jon W. Gordon, Joan Uehlinger, Nooshine Dayani, Beth E. Talansky, Mildred Gordon, Greg S. Rudomen, Paul E. Neumann

Research output: Contribution to journal › Article › peer-review

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Abstract

Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated ho^Jwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in ho^Jwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.

Original language	English (US)
Pages (from-to)	349-358
Number of pages	10
Journal	Developmental Biology
Volume	137
Issue number	2
DOIs	https://doi.org/10.1016/0012-1606(90)90259-L
State	Published - Feb 1990
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(90)90259-L

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@article{3d687b6b0704462ea19e50da4c16a483,

title = "Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse",

abstract = "Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated hoJwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in hoJwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.",

author = "Gordon, {Jon W.} and Joan Uehlinger and Nooshine Dayani and Talansky, {Beth E.} and Mildred Gordon and Rudomen, {Greg S.} and Neumann, {Paul E.}",

note = "Funding Information: We acknowledgeD rs. Philip Leder and Lydia Villa-Komaroff for their critical review of this manuscript. This work was supported by NIH Grants CA42103a nd HD20484a nd March of Dimes Grant l-1026 to J.W.G. and by an NIH Grant NS20820t o Dr. Richard Sidman. This is manuscript No. 16 of the Brookdale Center for Molecular Biology, Mt. Sinai Medical Center.",

year = "1990",

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doi = "10.1016/0012-1606(90)90259-L",

language = "English (US)",

volume = "137",

pages = "349--358",

journal = "Developmental Biology",

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T1 - Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

AU - Gordon, Jon W.

AU - Uehlinger, Joan

AU - Dayani, Nooshine

AU - Talansky, Beth E.

AU - Gordon, Mildred

AU - Rudomen, Greg S.

AU - Neumann, Paul E.

N1 - Funding Information: We acknowledgeD rs. Philip Leder and Lydia Villa-Komaroff for their critical review of this manuscript. This work was supported by NIH Grants CA42103a nd HD20484a nd March of Dimes Grant l-1026 to J.W.G. and by an NIH Grant NS20820t o Dr. Richard Sidman. This is manuscript No. 16 of the Brookdale Center for Molecular Biology, Mt. Sinai Medical Center.

PY - 1990/2

Y1 - 1990/2

N2 - Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated hoJwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in hoJwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.

AB - Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated hoJwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in hoJwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.

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Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

Abstract

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