Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

Jon W. Gordon, Joan Uehlinger, Nooshine Dayani, Beth E. Talansky, Mildred Gordon, Greg S. Rudomen, Paul E. Neumann

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated hoJwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in hoJwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.

Original languageEnglish (US)
Pages (from-to)349-358
Number of pages10
JournalDevelopmental Biology
Issue number2
StatePublished - Feb 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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