Analysis of hVps34/hVps15 interactions with Rab5 in Vivo and in Vitro

James T. Murray; Jonathan M. Backer

doi:10.1016/S0076-6879(05)03068-5

Analysis of hVps34/hVps15 interactions with Rab5 in Vivo and in Vitro

James T. Murray, Jonathan M. Backer

Molecular Pharmacology

Research output: Contribution to journal › Review article › peer-review

7 Scopus citations

Abstract

The hVps34 phosphatidylinositol (PI) 3-kinase plays an important role in the regulation of vesicular trafficking in the endosomal system. hVps34 associates with a myristylated protein kinase, hVps15. The two proteins are targeting to early endosomal membranes by interactions between hVps15 and activated (GTP-bound) Rab5. This leads to the production of the hVps34 product, PI(3)P, in the endosomal membrane, and subsequent recruitment of FYVE and PX domain-containing effector proteins. This chapter describes the analysis of hVps34/hVps15 interactions with Rab5 in tissue culture cells and in vitro.

Original language	English (US)
Article number	68
Pages (from-to)	789-799
Number of pages	11
Journal	Methods in enzymology
Volume	403
DOIs	https://doi.org/10.1016/S0076-6879(05)03068-5
State	Published - Jan 1 2005

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(05)03068-5

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title = "Analysis of hVps34/hVps15 interactions with Rab5 in Vivo and in Vitro",

abstract = "The hVps34 phosphatidylinositol (PI) 3-kinase plays an important role in the regulation of vesicular trafficking in the endosomal system. hVps34 associates with a myristylated protein kinase, hVps15. The two proteins are targeting to early endosomal membranes by interactions between hVps15 and activated (GTP-bound) Rab5. This leads to the production of the hVps34 product, PI(3)P, in the endosomal membrane, and subsequent recruitment of FYVE and PX domain-containing effector proteins. This chapter describes the analysis of hVps34/hVps15 interactions with Rab5 in tissue culture cells and in vitro.",

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N2 - The hVps34 phosphatidylinositol (PI) 3-kinase plays an important role in the regulation of vesicular trafficking in the endosomal system. hVps34 associates with a myristylated protein kinase, hVps15. The two proteins are targeting to early endosomal membranes by interactions between hVps15 and activated (GTP-bound) Rab5. This leads to the production of the hVps34 product, PI(3)P, in the endosomal membrane, and subsequent recruitment of FYVE and PX domain-containing effector proteins. This chapter describes the analysis of hVps34/hVps15 interactions with Rab5 in tissue culture cells and in vitro.

AB - The hVps34 phosphatidylinositol (PI) 3-kinase plays an important role in the regulation of vesicular trafficking in the endosomal system. hVps34 associates with a myristylated protein kinase, hVps15. The two proteins are targeting to early endosomal membranes by interactions between hVps15 and activated (GTP-bound) Rab5. This leads to the production of the hVps34 product, PI(3)P, in the endosomal membrane, and subsequent recruitment of FYVE and PX domain-containing effector proteins. This chapter describes the analysis of hVps34/hVps15 interactions with Rab5 in tissue culture cells and in vitro.

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Analysis of hVps34/hVps15 interactions with Rab5 in Vivo and in Vitro

Abstract

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