TY - JOUR
T1 - An in vitro culture system for long-term expansion of epithelial and mesenchymal salivary gland cells
T2 - Role of TGF- β 1 in salivary gland epithelial and mesenchymal differentiation
AU - Janebodin, Kajohnkiart
AU - Buranaphatthana, Worakanya
AU - Ieronimakis, Nicholas
AU - Hays, Aislinn L.
AU - Reyes, Morayma
PY - 2013
Y1 - 2013
N2 - Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used the Col1a1-GFP mouse model to characterize the salivary gland mesenchyme in vitro and in vivo. The Col1a1-GFP transgene was exclusively expressed in the salivary gland mesenchyme. Ex vivo culture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF- β 1 in salivary gland development and disease is complex. Therefore, we used this in vitro culture system to study the effects of TGF- β 1 on salivary gland cell differentiation. TGF- β 1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF- β R1 inhibition increased acini genes and fibroblast growth factors (Fgf-7 and Fgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF- β signaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF- β 1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors.
AB - Despite a pivotal role in salivary gland development, homeostasis, and disease, the role of salivary gland mesenchyme is not well understood. In this study, we used the Col1a1-GFP mouse model to characterize the salivary gland mesenchyme in vitro and in vivo. The Col1a1-GFP transgene was exclusively expressed in the salivary gland mesenchyme. Ex vivo culture of mixed salivary gland cells in DMEM plus serum medium allowed long-term expansion of salivary gland epithelial and mesenchymal cells. The role of TGF- β 1 in salivary gland development and disease is complex. Therefore, we used this in vitro culture system to study the effects of TGF- β 1 on salivary gland cell differentiation. TGF- β 1 induced the expression of collagen, and inhibited the formation of acini-like structures in close proximity to mesenchymal cells, which adapted a fibroblastic phenotype. In contrast, TGF- β R1 inhibition increased acini genes and fibroblast growth factors (Fgf-7 and Fgf-10), decreased collagen and induced formation of larger, mature acini-like structures. Thus, inhibition of TGF- β signaling may be beneficial for salivary gland differentiation; however, due to differential effects of TGF- β 1 in salivary gland epithelial versus mesenchymal cells, selective inhibition is desirable. In conclusion, this mixed salivary gland cell culture system can be used to study epithelial-mesenchymal interactions and the effects of differentiating inducers and inhibitors.
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U2 - 10.1155/2013/815895
DO - 10.1155/2013/815895
M3 - Article
C2 - 23841093
AN - SCOPUS:84880154193
SN - 2314-6133
VL - 2013
JO - BioMed Research International
JF - BioMed Research International
M1 - 815895
ER -