Abstract
Monospecific anti-[4-(N-maleimidobenzyl)trimethylammonium] (MBTA) antibodies were prepared from sera of rabbits immunized with an albumin-MBTA conjugate and used to synthesize an MBTA-specific immunosorbent. Torpedo californica acetylcholine receptor was affinity labeled with [3H]-MBTA and proteolyzed extensively with pronase, and the peptide fraction of the digest chromatographed on the anti-MBTA resin. The amino acid composition of the purified MBTA-peptide fraction was compared with the sequences flanking the seven cysteinyl residues of the α-subunit. The best fit was observed with the segment containing cysteine 142.
Original language | English (US) |
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Pages (from-to) | 602-608 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 122 |
Issue number | 2 |
DOIs | |
State | Published - Jul 31 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology