A toll-like receptor 9 (rs352140) variant is associated with placental inflammation in newborn infants

Vijender Karody, Shawn Reese, Navin Kumar, Jennifer Liedel, Jason Jarzembowski, Venkatesh Sampath

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Objective: Chorioamnionitis contributes to premature birth and associated postnatal morbidity. The genetic basis of altered immune responses underlying placental inflammation (PI) remains understudied. The aim of this study was to evaluate the relationship among TLR signaling pathway polymorphisms and different patterns of PI.Methods: Prospective cohort study in infants involving cord blood collection and placental examination for PI. One hundred and fifty-nine infants enrolled in study out of which 28 were term (eight with PI) and 131 preterm (47 with PI). DNA from blood was genotyped for SNPs in TLR2, 4, 5, 9, NFKBI, NFKBIA, TIRAP, and IRAK1 genes using multiplexed single base extension assay.Results: While there were no differences in BW, GA, gender, race, and SPL among infants with or without PI, there was a higher incidence of PPROM, maternal smoking, drug use, and clinical chorioamnionitis among infants with PI. Out of nine TLR variants, only CT and/or TT genotypes of the TLR9 variant (rs352140) were significantly associated (p = 0.004) with any PI and maternal pattern of inflammation (p = 0.012) both by univariate analysis and logistic regression.Conclusions: The presence of a variant T allele in a common SNP (rs352140) in the TLR9 gene whose product recognizes bacterial DNA is associated with increased PI.

Original languageEnglish (US)
Pages (from-to)2210-2216
Number of pages7
JournalJournal of Maternal-Fetal and Neonatal Medicine
Issue number13
StatePublished - Jul 2 2016


  • Chorioamnionitis
  • innate immunity
  • placental inflammation
  • rs352140
  • toll-like receptor 9 polymorphisms

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology


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