@article{8be860653c7a4e1d82fa2bfde369b80a,
title = "A TAF4-homology domain from the corepressor ETO is a docking platform for positive and negative regulators of transcription",
abstract = "The eight twenty-one protein, ETO, is implicated in 12%-15% of acute human leukemias as part of a gene fusion with RUNX1 (also called AML1). Of the four ETO domains related to Drosophila melanogaster Nervy, only two are required to induce spontaneous myeloid leukemia upon transplantation into the mouse. One of these domains is related in sequence to TAF4, a component of TFIID. The structure of this domain, ETO-TAFH, is similar to yeast Rpb4 and to Escherichia coli σ70; it is the first TAF-related protein with structural similarity to the multisubunit RNA polymerases. Overlapping surfaces of ETO-TAFH interact with an autonomous repression domain of the nuclear receptor corepressor N-CoR and with a conserved activation domain from the E-box family of transcription factors. Thus, ETO-TAFH acts as a structural platform that can interchange negative and positive coregulatory proteins to control transcription.",
author = "Yufeng Wei and Shaohua Liu and J{\"o}rn Lausen and Christopher Woodrell and Seongeun Cho and Nikolaos Biris and Naohiro Kobayashi and Yu Wei and Shigeyuki Yokoyama and Werner, {Milton H.}",
note = "Funding Information: This work was supported in part by a fellowship from the Deutsche Forschungsgemeinschaft (LA 1389/1-1 to J.L.), by the Specialized Center of Research Grant from the Leukemia and Lymphoma Society (to M.H.W.) and by the RIKEN Structural Genomics/Proteomics Initiative, the National Project on Protein Structural and Functional Analyses, Ministry of Education, Culture, Sports, Science and Technology of Japan. M.H.W. is a Distinguished Young Scholar of the W.M. Keck Foundation. We thank S. Hiebert (Vanderbilt University), N. Timchenko (Baylor College of Medicine), E.P. Reddy (Temple University), M.J. Klemsz (Indiana University) and S. Nimer (Memorial Sloan-Kettering Cancer Center) for providing expression and reporter vectors, T. Berg and M. L{\"u}bbert (University of Freiberg) for sharing AML1-ETO microarray data before publication and M. Punta and B. Rost for sequence analysis of the N-CoR– and E-protein–binding motifs.",
year = "2007",
month = jul,
doi = "10.1038/nsmb1258",
language = "English (US)",
volume = "14",
pages = "653--661",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "7",
}