A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Yee Guide Yeung; E. Richard Stanley

doi:10.1016/j.ab.2009.03.017

A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Yee Guide Yeung, E. Richard Stanley

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

Original language	English (US)
Pages (from-to)	89-91
Number of pages	3
Journal	Analytical Biochemistry
Volume	389
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2009.03.017
State	Published - Jun 1 2009

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing",

abstract = "Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.",

author = "Yeung, {Yee Guide} and Stanley, {E. Richard}",

note = "Funding Information: This work was supported by National Institutes of Health grant CA26504 (to E.R.S.) and Albert Einstein College of Medicine Cancer Center grant 5P30-CA13330.",

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N2 - Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

AB - Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

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A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Abstract

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