A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Yee Guide Yeung; E. Richard Stanley

doi:10.1016/j.ab.2009.03.017

A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Yee Guide Yeung, E. Richard Stanley

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

Original language	English (US)
Pages (from-to)	89-91
Number of pages	3
Journal	Analytical Biochemistry
Volume	389
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2009.03.017
State	Published - Jun 1 2009
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.ab.2009.03.017

Cite this

@article{710454c2611c4706b7207fe44dec5ebf,

title = "A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing",

abstract = "Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.",

author = "Yeung, {Yee Guide} and Stanley, {E. Richard}",

note = "Funding Information: This work was supported by National Institutes of Health grant CA26504 (to E.R.S.) and Albert Einstein College of Medicine Cancer Center grant 5P30-CA13330.",

year = "2009",

month = jun,

day = "1",

doi = "10.1016/j.ab.2009.03.017",

language = "English (US)",

volume = "389",

pages = "89--91",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

AU - Yeung, Yee Guide

AU - Stanley, E. Richard

N1 - Funding Information: This work was supported by National Institutes of Health grant CA26504 (to E.R.S.) and Albert Einstein College of Medicine Cancer Center grant 5P30-CA13330.

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

AB - Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

UR - http://www.scopus.com/inward/record.url?scp=65049085779&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65049085779&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2009.03.017

DO - 10.1016/j.ab.2009.03.017

M3 - Article

C2 - 19303392

AN - SCOPUS:65049085779

SN - 0003-2697

VL - 389

SP - 89

EP - 91

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this