A role for ferrous ion and oxygen in the degradation of DNA by bleomycin

Edward A. Sausville; Jack Peisach; Susan B. Horwitz

doi:10.1016/0006-291X(76)90882-2

A role for ferrous ion and oxygen in the degradation of DNA by bleomycin

Edward A. Sausville
, Jack Peisach
, Susan B. Horwitz

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

286 Scopus citations

Abstract

An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported. Complete conversion of simian virus 40 DNA to acid-soluble products occurs at approximately equimolar levels of Fe(II), bleomycin, and DNA; Fe(III) does not substitute for Fe(II) in this reaction. Anaerobiosis inhibits the observed DNA degradation by bleomycin and Fe(II). Optical spectral studies reveal that an oxygen-labile complex is formed between bleomycin and Fe(II).

Original language	English (US)
Pages (from-to)	814-822
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(76)90882-2
State	Published - Dec 6 1976

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(76)90882-2

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abstract = "An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported. Complete conversion of simian virus 40 DNA to acid-soluble products occurs at approximately equimolar levels of Fe(II), bleomycin, and DNA; Fe(III) does not substitute for Fe(II) in this reaction. Anaerobiosis inhibits the observed DNA degradation by bleomycin and Fe(II). Optical spectral studies reveal that an oxygen-labile complex is formed between bleomycin and Fe(II).",

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AU - Peisach, Jack

AU - Horwitz, Susan B.

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Y1 - 1976/12/6

N2 - An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported. Complete conversion of simian virus 40 DNA to acid-soluble products occurs at approximately equimolar levels of Fe(II), bleomycin, and DNA; Fe(III) does not substitute for Fe(II) in this reaction. Anaerobiosis inhibits the observed DNA degradation by bleomycin and Fe(II). Optical spectral studies reveal that an oxygen-labile complex is formed between bleomycin and Fe(II).

AB - An interaction between bleomycin and low concentrations of Fe(II) in the degradation of DNA is reported. Complete conversion of simian virus 40 DNA to acid-soluble products occurs at approximately equimolar levels of Fe(II), bleomycin, and DNA; Fe(III) does not substitute for Fe(II) in this reaction. Anaerobiosis inhibits the observed DNA degradation by bleomycin and Fe(II). Optical spectral studies reveal that an oxygen-labile complex is formed between bleomycin and Fe(II).

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U2 - 10.1016/0006-291X(76)90882-2

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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A role for ferrous ion and oxygen in the degradation of DNA by bleomycin

Abstract

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