A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system

Winfried Edelmann; Burkhard Kröger; Martin Goller; Ivan Horak

doi:10.1016/0092-8674(89)90332-2

A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system

Winfried Edelmann, Burkhard Kröger, Martin Goller, Ivan Horak

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.

Original language	English (US)
Pages (from-to)	937-946
Number of pages	10
Journal	Cell
Volume	57
Issue number	6
DOIs	https://doi.org/10.1016/0092-8674(89)90332-2
State	Published - Jun 16 1989
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system",

abstract = "The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.",

author = "Winfried Edelmann and Burkhard Kr{\"o}ger and Martin Goller and Ivan Horak",

note = "Funding Information: We thank Dr. S. Hiinig for the synthesis of bromoacetaldehyde, Drs. I. Grummt, E. Serfling, and S. Siddell for stimulating discussion and helpful comments on the manuscript, and Carmen Strasser and Andrea Peters for excellent technical assistance. This work was supported by grants from Deutsche Forschungsgemeinschaft (SFB 165) and from the Fonds der Chemischen Industrie.",

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doi = "10.1016/0092-8674(89)90332-2",

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AU - Edelmann, Winfried

AU - Kröger, Burkhard

AU - Goller, Martin

AU - Horak, Ivan

N1 - Funding Information: We thank Dr. S. Hiinig for the synthesis of bromoacetaldehyde, Drs. I. Grummt, E. Serfling, and S. Siddell for stimulating discussion and helpful comments on the manuscript, and Carmen Strasser and Andrea Peters for excellent technical assistance. This work was supported by grants from Deutsche Forschungsgemeinschaft (SFB 165) and from the Fonds der Chemischen Industrie.

PY - 1989/6/16

Y1 - 1989/6/16

N2 - The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.

AB - The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.

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A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system

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