A pilot analytic study of a research-level, lower-cost human papillomavirus 16, 18, and 45 test

Hannah P. Yang; David K. Walmer; Delson Merisier; Julia C. Gage; Laura Bell; Sameera Rangwala; Niwashin Shrestha; Lori Kobayashi; Paul S. Eder; Philip E. Castle

doi:10.1016/j.jviromet.2011.05.024

A pilot analytic study of a research-level, lower-cost human papillomavirus 16, 18, and 45 test

Hannah P. Yang
, David K. Walmer
, Delson Merisier
, Julia C. Gage
, Laura Bell
, Sameera Rangwala
, Niwashin Shrestha
, Lori Kobayashi
, Paul S. Eder
, Philip E. Castle

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The analytic performance of a low-cost, research-stage DNA test for the most carcinogenic human papillomavirus (HPV) genotypes (HPV16, HPV18, and HPV45) in aggregate was evaluated among carcinogenic HPV-positive women, which might be used to decide who needs immediate colposcopy in low-resource settings (" triage test"). We found that HPV16/18/45 test agreed well with two DNA tests, a GP5+/6+ genotyping assay (Kappa = 0.77) and a quantitative PCR assay (at a cutpoint of 5000 viral copies) (Kappa = 0.87). DNA sequencing on a subset of 16 HPV16/18/45 positive and 16 HPV16/18/45 negative verified the analytic specificity of the research test. It is concluded that the HPV16/18/45 assay is a promising triage test with a minimum detection of approximately 5000 viral copies, the clinically relevant threshold.

Original language	English (US)
Pages (from-to)	112-114
Number of pages	3
Journal	Journal of Virological Methods
Volume	176
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2011.05.024
State	Published - Sep 2011
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cervical cancer
HPV genotypes
HPV test
Human papillomavirus (HPV)

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2011.05.024

Cite this

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title = "A pilot analytic study of a research-level, lower-cost human papillomavirus 16, 18, and 45 test",

abstract = "The analytic performance of a low-cost, research-stage DNA test for the most carcinogenic human papillomavirus (HPV) genotypes (HPV16, HPV18, and HPV45) in aggregate was evaluated among carcinogenic HPV-positive women, which might be used to decide who needs immediate colposcopy in low-resource settings ({"} triage test{"}). We found that HPV16/18/45 test agreed well with two DNA tests, a GP5+/6+ genotyping assay (Kappa = 0.77) and a quantitative PCR assay (at a cutpoint of 5000 viral copies) (Kappa = 0.87). DNA sequencing on a subset of 16 HPV16/18/45 positive and 16 HPV16/18/45 negative verified the analytic specificity of the research test. It is concluded that the HPV16/18/45 assay is a promising triage test with a minimum detection of approximately 5000 viral copies, the clinically relevant threshold.",

keywords = "Cervical cancer, HPV genotypes, HPV test, Human papillomavirus (HPV)",

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note = "Funding Information: The evaluation of the HPV16/18/45 triage test data was conducted for research purposes and was supported in part by the intramural research program of the NIH/NCI . The authors would like to recognize Adam Mallonee of QIAGEN for his contribution to performing the genotyping tests.",

year = "2011",

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language = "English (US)",

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AU - Walmer, David K.

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AU - Bell, Laura

AU - Rangwala, Sameera

AU - Shrestha, Niwashin

AU - Kobayashi, Lori

AU - Eder, Paul S.

AU - Castle, Philip E.

N1 - Funding Information: The evaluation of the HPV16/18/45 triage test data was conducted for research purposes and was supported in part by the intramural research program of the NIH/NCI . The authors would like to recognize Adam Mallonee of QIAGEN for his contribution to performing the genotyping tests.

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A pilot analytic study of a research-level, lower-cost human papillomavirus 16, 18, and 45 test

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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