A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

Gerald Cohen; Mimi Kim; Vivian Ogwu

doi:10.1016/0165-0270(96)00011-8

A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

Gerald Cohen, Mimi Kim, Vivian Ogwu

Research output: Contribution to journal › Article › peer-review

104 Scopus citations

Abstract

The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader and for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.

Original language	English (US)
Pages (from-to)	53-56
Number of pages	4
Journal	Journal of Neuroscience Methods
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/0165-0270(96)00011-8
State	Published - Jul 1996
Externally published	Yes

Keywords

Catalase assay
Cell culture
Mesencephalon
Plate reader

ASJC Scopus subject areas

General Neuroscience

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10.1016/0165-0270(96)00011-8

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title = "A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures",

abstract = "The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader and for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.",

keywords = "Catalase assay, Cell culture, Mesencephalon, Plate reader",

author = "Gerald Cohen and Mimi Kim and Vivian Ogwu",

note = "Funding Information: Supported, in part, by a research grant from the National Institutes of Health (NS-23017) and by a Fellowship to M.K. from the Parkinson{\textquoteright}s Disease Foundation. We thank Shan-Kuo Han for providing the cell cultures.",

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T1 - A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

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AU - Kim, Mimi

AU - Ogwu, Vivian

N1 - Funding Information: Supported, in part, by a research grant from the National Institutes of Health (NS-23017) and by a Fellowship to M.K. from the Parkinson’s Disease Foundation. We thank Shan-Kuo Han for providing the cell cultures.

PY - 1996/7

Y1 - 1996/7

N2 - The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader and for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.

AB - The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader and for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.

KW - Catalase assay

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KW - Mesencephalon

KW - Plate reader

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A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

Abstract

Keywords

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