A model system for analyzing somatic mutations in Drosophila melanogaster

Ana Maria Garcia; Anastasia Derventzi; Rita Busuttil; R. Brent Calder; Ernesto Perez; Linda Chadwell; Martijn E.T. Dollé; Martha Lundell; Jan Vijg

doi:10.1038/nmeth1027

A model system for analyzing somatic mutations in Drosophila melanogaster

Ana Maria Garcia, Anastasia Derventzi, Rita Busuttil, R. Brent Calder, Ernesto Perez, Linda Chadwell, Martijn E.T. Dollé, Martha Lundell, Jan Vijg

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.

Original language	English (US)
Pages (from-to)	401-403
Number of pages	3
Journal	Nature Methods
Volume	4
Issue number	5
DOIs	https://doi.org/10.1038/nmeth1027
State	Published - May 2007
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth1027

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title = "A model system for analyzing somatic mutations in Drosophila melanogaster",

abstract = "Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.",

author = "Garcia, {Ana Maria} and Anastasia Derventzi and Rita Busuttil and Calder, {R. Brent} and Ernesto Perez and Linda Chadwell and Doll{\'e}, {Martijn E.T.} and Martha Lundell and Jan Vijg",

note = "Funding Information: We thank P. Kapahi for critical comments and valuable suggestions. This work was supported by a grant from the US National Institutes of Health (AG20438).",

year = "2007",

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T1 - A model system for analyzing somatic mutations in Drosophila melanogaster

AU - Garcia, Ana Maria

AU - Derventzi, Anastasia

AU - Busuttil, Rita

AU - Calder, R. Brent

AU - Perez, Ernesto

AU - Chadwell, Linda

AU - Dollé, Martijn E.T.

AU - Lundell, Martha

AU - Vijg, Jan

N1 - Funding Information: We thank P. Kapahi for critical comments and valuable suggestions. This work was supported by a grant from the US National Institutes of Health (AG20438).

PY - 2007/5

Y1 - 2007/5

N2 - Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.

AB - Presently there are no good assays for comparing somatic mutation frequencies and spectra between different vertebrate and invertebrate organisms. Here we describe a new lacZ mutation reporter system in D. melanogaster, which complements existing systems in the mouse. The results obtained with the new model indicate two-to threefold higher frequencies of spontaneous mutations than in the mouse, with most of the mutations characterized as large genome rearrangements.

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A model system for analyzing somatic mutations in Drosophila melanogaster

Abstract

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