Abstract
Abstract
Centenarians provide a unique lens through which to study longevity, healthy aging,
and resiliency. Moreover, models of human aging and resilience to disease that allow
for the testing of potential interventions are virtually non-existent. We obtained and
characterized over 96 centenarian and offspring peripheral blood samples including
those connected to functional independence data highlighting resistance to disability
and cognitive impairment. Targeted methylation arrays were used in molecular aging
clocks to compare and contrast differences between biological and chronological age
in these specialized subjects. Isolated peripheral blood mononuclear cells (PBMCs)
from 20 of these subjects were then successfully reprogrammed into high-quality
induced pluripotent stem cell (iPSC) lines which were functionally characterized for
pluripotency, genomic stability, and the ability to undergo directed differentiation.
The result of this work is a one-of-a-kind resource for studies of human longevity and
resilience that can fuel the discovery and validation of novel therapeutics for agingrelated disease.
Centenarians provide a unique lens through which to study longevity, healthy aging,
and resiliency. Moreover, models of human aging and resilience to disease that allow
for the testing of potential interventions are virtually non-existent. We obtained and
characterized over 96 centenarian and offspring peripheral blood samples including
those connected to functional independence data highlighting resistance to disability
and cognitive impairment. Targeted methylation arrays were used in molecular aging
clocks to compare and contrast differences between biological and chronological age
in these specialized subjects. Isolated peripheral blood mononuclear cells (PBMCs)
from 20 of these subjects were then successfully reprogrammed into high-quality
induced pluripotent stem cell (iPSC) lines which were functionally characterized for
pluripotency, genomic stability, and the ability to undergo directed differentiation.
The result of this work is a one-of-a-kind resource for studies of human longevity and
resilience that can fuel the discovery and validation of novel therapeutics for agingrelated disease.
| Original language | English (US) |
|---|---|
| Article number | 14351 |
| Pages (from-to) | 1-13 |
| Number of pages | 13 |
| Journal | Aging Cell |
| State | Published - Sep 25 2024 |
Keywords
- centenarians, induced pluripotent stem cells, longevity
