A dominant positive and negative selectable gene for use in mammalian cells

Faina Schwartz, Nobuyo Maeda, Oliver Smithies, Robert Hickey, Winfried Edelmann, Arthur Skoultchi, Raju Kucherlapati

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells. (.

Original languageEnglish (US)
Pages (from-to)10416-10420
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number23
StatePublished - Dec 1 1991


  • Bacterial neomycin phosphotransferase
  • Gene fusionh
  • Herpes simplex virus thymidine kinase

ASJC Scopus subject areas

  • General


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