TY - JOUR
T1 - 1′-Acetoxychavicol acetate induces apoptosis of myeloma cells via induction of TRAIL
AU - Ito, Keisuke
AU - Nakazato, Tomonori
AU - Murakami, Akira
AU - Ohigashi, Hajime
AU - Ikeda, Yasuo
AU - Kizaki, Masahiro
N1 - Funding Information:
We thank Ms. Kaori Saito for her excellent technical assistance. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan; the Takeda Science Foundation; and the Mitsubishi Pharma Research Foundation.
PY - 2005/12/30
Y1 - 2005/12/30
N2 - A component of a traditional Thai condiment, 1′-acetoxychavicol acetate (ACA), is a natural compound, and it is obtained from rhizomes of the ethno-medicinal plant Languas galanga (Zingiberaceae). Our previous studies showed that ACA dramatically inhibited cellular growth of multiple myeloma cells in vivo and in vitro through the induction of apoptosis in association with the activation of caspase-8, inactivation of NF-κB, and down-regulation of anti-apoptotic proteins. Subsequently, we investigated the detailed apoptotic pathway of ACA and further demonstrated that ACA up-regulates the expression of both TNF-related apoptosis-inducing ligand/Apo2 ligand (TRAIL/Apo2L) and TRAIL receptor death receptor 5 (DR5). In addition, TRAIL/R-Fc chimera neutralizes the ACA-induced apoptosis. These results suggest that the death signaling of TRAIL is involved in the ACA-induced apoptosis of myeloma cells, and provide a rationale for the induction of TRAIL/Apo2L by ACA, which could potentially be used as a novel therapeutic agent in patients with multiple myeloma.
AB - A component of a traditional Thai condiment, 1′-acetoxychavicol acetate (ACA), is a natural compound, and it is obtained from rhizomes of the ethno-medicinal plant Languas galanga (Zingiberaceae). Our previous studies showed that ACA dramatically inhibited cellular growth of multiple myeloma cells in vivo and in vitro through the induction of apoptosis in association with the activation of caspase-8, inactivation of NF-κB, and down-regulation of anti-apoptotic proteins. Subsequently, we investigated the detailed apoptotic pathway of ACA and further demonstrated that ACA up-regulates the expression of both TNF-related apoptosis-inducing ligand/Apo2 ligand (TRAIL/Apo2L) and TRAIL receptor death receptor 5 (DR5). In addition, TRAIL/R-Fc chimera neutralizes the ACA-induced apoptosis. These results suggest that the death signaling of TRAIL is involved in the ACA-induced apoptosis of myeloma cells, and provide a rationale for the induction of TRAIL/Apo2L by ACA, which could potentially be used as a novel therapeutic agent in patients with multiple myeloma.
KW - 1′-Acetoxychavicol acetate
KW - Apoptosis
KW - Multiple myeloma
KW - TRAIL
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U2 - 10.1016/j.bbrc.2005.10.153
DO - 10.1016/j.bbrc.2005.10.153
M3 - Article
C2 - 16289031
AN - SCOPUS:27844602024
SN - 0006-291X
VL - 338
SP - 1702
EP - 1710
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -