Engineering Sortase Variants for Intracellular and Cell Surface Labeling

  • Levy, Matthew (PI)

Project: Research project

Project Details


DESCRIPTION (provided by applicant): We propose to develop a new system for site specific labeling proteins in live cells based on the bacterial transpeptidase sortase A (SrtA). Using a combination of protein engineering and directed evolution, we will develop SrtA variants capable of efficiently functioning within mammalian cells. We will also develop exceptionally small (
Effective start/end date9/30/098/31/15


  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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